Computational screening and molecular dynamics simulation of disease associated nsSNPs in CENP-E

被引:61
作者
Kumar, Ambuj [1 ]
Purohit, Rituraj [1 ]
机构
[1] Vellore Inst Technol Univ, Sch Bio Sci & Technol, Bioinformat Div, Vellore 632014, Tamil Nadu, India
关键词
CENP-E protein; Cancer; Molecular dynamics simulation; SNP analysis; HIV-1 PROTEASE MUTANTS; MITOTIC CHECKPOINT; MICROTUBULE CAPTURE; CANCER; KINETOCHORE; ANEUPLOIDY; PROTEINS; EXPRESSION; CARCINOMA; CELLS;
D O I
10.1016/j.mrfmmm.2012.08.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aneuploidy and chromosomal instability (CIN) are hallmarks of most solid tumors. Mutations in centroemere proteins have been observed in promoting aneuploidy and tumorigenesis. Recent studies reported that Centromere-associated protein-E(CENP-E) is involved in inducing cancers. In this study we investigated the pathogenic effect of 132 nsSNPs reported in CENP-E using computational platform. Y63H point mutation found to be associated with cancer using SIFT, Polyphen, PhD-SNP, MutPred, CanPredict and Dr. Cancer tools. Further we investigated the binding affinity of ATP molecule to the CENP-E motor domain. Complementarity scores obtained from docking studies showed significant loss in ATP binding affinity of mutant structure. Molecular dynamics simulation was carried to examine the structural consequences of Y63H mutation. Root mean square deviation (RMSD), root mean square fluctuation (RMSF), radius of gyration (R-g), solvent accessibility surface area (SASA), energy value, hydrogen bond (NH Bond), eigenvector projection, trace of covariance matrix and atom density analysis results showed notable loss in stability for mutant structure. Y63H mutation was also shown to disrupt the native conformation of ATP binding region in CENP-E motor domain. Docking studies for remaining 18 mutations at 63rd residue position as well as other two computationally predicted disease associated mutations S22L and P695 were also carried to investigate their affect on ATP binding affinity of CENP-E motor domain. Our study provided a promising computational methodology to study the tumorigenic consequences of nsSNPs that have not been characterized and clear clue to the wet lab scientist. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:28 / 37
页数:10
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