SARS-CoV-2 Spatiotemporal Genomic and Molecular Analysis of the First Wave of the COVID-19 Pandemic in Macae, the Brazilian Capital of Oil

被引：1

作者：

da-Costa-Rodrigues, Bruno ^{[1
,2
]}

Cheohen, Caio ^{[1
]}

Sciammarella, Felipe ^{[3
]}

Pierre-Bonetti-Pozzobon, Allan ^{[1
]}

Ribeiro, Lupis ^{[1
]}

Nepomuceno-Silva, Jose Luciano ^{[1
]}

Medeiros, Marcio ^{[1
]}

Mury, Flavia ^{[1
]}

Monteiro-de-Barros, Cintia ^{[1
]}

Lazoski, Cristiano ^{[3
]}

Leal-da-Silva, Manuela ^{[1
]}

Tanuri, Amilcar ^{[3
]}

Nunes-da-Fonseca, Rodrigo ^{[1
,2
]}

机构：

[1] Univ Fed Rio de Janeiro UFRJ, Inst Biodiversidade & Sustentabilidade NUPEM, Av Sao Jose do Barreto 764, BR-27965550 Macae, Brazil

[2] Univ Fed Rio de Janeiro UFRJ, Inst Ciencias Biomed, Programa Ciencias Morfol, BR-21941170 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro UFRJ, Inst Biol, BR-21941902 Rio De Janeiro, Brazil

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 19期

关键词：

SARS-CoV-2; COVID-19; epidemiology; Macae; Brazil; MUTATIONS; DYNAMICS;

D O I：

10.3390/ijms231911497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SARS-CoV-2 virus infection led to millions of deaths during the COVID-19 pandemic. Hundreds of workers from several other Brazilian cities, as well as from other countries, arrive daily in Macae to work in the oil supply chain, making this city a putative hotspot for the introduction of new viral lineages. In this study, we performed a genomic survey of SARS-CoV-2 samples from Macae during the first outbreak of COVID-19, combined with clinical data and a molecular integrative analysis. First, phylogenomic analyses showed a high occurrence of viral introduction events and the establishment of local transmissions in Macae, including the ingression and spread of the B.1.1.28 lineage in the municipality from June to August 2020. Second, SARS-CoV-2 mutations were identified in patients with distinct levels of COVID-19 severity. Third, molecular interactions of the mutated spike protein from three B.1.1.33 local samples and human ACE2 showed higher interactions than that of the wild-type spike protein from the ancestral virus. Altogether, these results elucidate the SARS-CoV-2 genomic profile in a strategic Brazilian city and further explore the functional aspects of SARS-CoV-2 with a characterization of emerging viral mutations associated with clinical data and the potential targets for drug development against SARS-CoV-2.

引用

页数：14

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