Bleaching of plasmon-resonance absorption of gold nanorods decreases efficiency of cell destruction

被引：16

作者：

Rudnitzki, Florian ^{[1
]}

Bever, Marco ^{[2
]}

Rahmanzadeh, Ramtin ^{[1
]}

Brieger, Katrin ^{[1
]}

Endl, Elmar ^{[3
,4
]}

Groll, Juergen ^{[5
]}

Huettmann, Gereon ^{[1
,2
]}

机构：

[1] Med Univ Lubeck, Inst Biomed Opt, D-23562 Lubeck, Germany

[2] Med Laser Ctr Lubeck MLL GmbH, D-23562 Lubeck, Germany

[3] Univ Bonn, Inst Mol Med, D-53105 Bonn, Germany

[4] Univ Bonn, Inst Expt Immunol, D-53105 Bonn, Germany

[5] Univ Wurzburg, Sch Med, Dept Funct Mat Med & Dent, D-97070 Wurzburg, Germany

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 05期

关键词：

nanoparticles; nanorods; laser nanoeffects; cavitation; transient bleaching; OPTICAL-PROPERTIES; NANOPARTICLES; SPECTROSCOPY; DYNAMICS; DEATH; SHAPE;

D O I：

10.1117/1.JBO.17.5.058003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the optical window of tissue. Thus, an increased efficiency of cell killing is expected with laser pulses tuned to the near infrared absorption peak of the nanorods. In contrast to the higher-absorption, experiments showed a reduced efficacy of cell killing. In order to explain this discrepancy, transient absorption of irradiated nanorods was measured and the observed change of particle absorption was theoretically analyzed. During pulsed irradiation a strong transient and permanent bleaching of the near-infrared absorption band occurred. Both effects limit the ability of nanorods to destroy cells by nanocavitation. The existence of nanocavitation and transient bleaching was corroborated by optoacoustic measurements. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.5.058003]

引用

页数：13

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