Enzymatic Direct Electron Transfer at Ascorbate Oxidase-modified Gold Electrode Prepared by One-step Galvanostatic Method

被引：0

作者：

Patil, Bhushan ^{[1
,2
]}

Fujikawa, Shigenori ^{[2
]}

Okajima, Takeyoshi ^{[1
]}

Ohsaka, Takeo ^{[1
]}

机构：

[1] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Elect Chem, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] RIKEN, Inst Phys & Chem Res, Interfacial Nanostruct Res Lab, Wako, Saitama 3510198, Japan

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2012年 / 7卷 / 06期

关键词：

Ascorbate oxidase; Direct electron transfer; Galvanostatic immobilization; TRAMETES-HIRSUTA LACCASE; BILIRUBIN OXIDASE; GLUCOSE-OXIDASE; 4-ELECTRON REDUCTION; DIOXYGEN; GRAPHITE; IMMOBILIZATION; DEPOSITION; BIOSENSOR; BARE;

D O I：

暂无

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A one-step enzyme immobilization by galvanostatic method at gold electrode was applied to achieve direct electron transfer (DET) of ascorbate oxidase from Acremonium sp. (ASOM). The enzyme immobilization was optimized and ASOM was successfully deposited on the gold electrode surface, typically by flowing the oxidation current of 6 mu A corresponding to oxygen evolution for 4 min, which results in a decrease in the local pH in the vicinity of the electrode surface and the precipitation of ASOM on the electrode surface due to the neutralization of its surface charge. A well-defined redox wave with a formal potential of 242 mV (vs. Ag vertical bar AgCl vertical bar KCl(sat)) was observed in 5.0 mM phosphate buffer solution (pH 7.0), confirming DET between ASOM and gold electrode surface.

引用

页码：5012 / 5019

页数：8

共 37 条

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