Hispolon Cyclodextrin Complexes and Their Inclusion in Liposomes for Enhanced Delivery in Melanoma Cell Lines

被引:9
|
作者
Poudel, Ishwor [1 ]
Annaji, Manjusha [1 ]
Wibowo, Fajar Setyo [1 ]
Arnold, Robert D. [1 ]
Fasina, Oladiran [2 ]
Via, Brian [3 ]
Rangari, Vijaya [4 ]
Peresin, Maria Soledad [5 ]
Smith, Forrest [1 ]
Dhanasekaran, Muralikrishnan [1 ]
Tiwari, Amit K. [6 ]
Babu, R. Jayachandra [1 ]
机构
[1] Auburn Univ, Harrison Coll Pharm, Dept Drug Discovery & Dev, Auburn, AL 36849 USA
[2] Auburn Univ, Dept Biosyst Engn, Auburn, AL 36849 USA
[3] Auburn Univ, Forest Prod Dev Ctr, Auburn, AL 36849 USA
[4] Tuskegee Univ, Dept Mat Sci Engn, Tuskegee, AL 36088 USA
[5] Auburn Univ, Coll Forestry, Forest Prod Dev Ctr, Sustainable Biobased Mat Lab, 602 Duncan Dr, Auburn, AL 36849 USA
[6] Univ Toledo, Dept Pharmacol & Expt Therapeut, Hlth Sci Campus,3000 Arlington Ave, Toledo, OH 43614 USA
关键词
hispolon; cyclodextrins; SBE beta CD; melanoma; cyclodextrins in liposomes; IN-LIPOSOMES; SYSTEM; NANOPARTICLES; ENCAPSULATION; INTEGRITY; CURCUMIN;
D O I
10.3390/ijms232214487
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hispolon, a phenolic pigment isolated from the mushroom species Phellinus linteus, has been investigated for anti-inflammatory, antioxidant, and anticancer properties; however, low solubility and poor bioavailability have limited its potential clinical translation. In this study, the inclusion complex of hispolon with Sulfobutylether-beta-cyclodextrin (SBE beta CD) was characterized, and the Hispolon-SBE beta CD Complex (HSC) was included within the sterically stabilized liposomes (SL) to further investigate its anticancer activity against melanoma cell lines. The HSC-trapped-Liposome (HSC-SL) formulation was investigated for its sustained drug delivery and enhanced cytotoxicity. The inclusion complex in the solid=state was confirmed by a Job's plot analysis, molecular modeling, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Proton nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). The HSCSL showed no appreciable deviation in size (<150 nm) and polydispersity index (<0.2) and improved drug encapsulation efficiency (>90%) as compared to control hispolon liposomes. Individually incorporated hispolon and SBE beta CD in the liposomes (H-CD-SL) was not significant in loading the drug in the liposomes, compared to HSC-SL, as a substantial amount of free drug was separated during dialysis. The HSC-SL formulation showed a sustained release compared to hispolon liposomes (H-SLs) and Hispolon-SBE beta CD liposomes (H-CD-SLs). The anticancer activity on melanoma cell lines (B16BL6) of HSC and HSC-SL was higher than in H-CD-SL and hispolon solution. These findings suggest that HSC inclusion in the HSC-SL liposomes stands out as a potential formulation approach for enhancing drug loading, encapsulation, and chemotherapeutic efficiency of hispolon and similar water insoluble drug molecules.
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页数:23
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