Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: Preliminary study

被引：13

作者：

Feuillet-Fieux, Marie-Noelle ^{[5
]}

Thao Nguyen-Khoa ^{[5
]}

Loriot, Marie-Anne ^{[1
,2
]}

Kelly, Mairead ^{[5
]}

de Villartay, Philippe ^{[3
]}

Sermet, Isabelle ^{[3
]}

Verrier, Patricia ^{[3
]}

Bonnefont, Jean-Paul ^{[4
]}

Beaune, Philippe ^{[1
,2
]}

Lenoir, Gerard ^{[3
]}

Lacour, Bernard ^{[5
]}

机构：

[1] Hop Europeen Georges Pompidou, Biochem Lab, F-75015 Paris, France

[2] Univ Paris 05, INSERM, U775, F-75006 Paris, France

[3] Hop Necker Enfants Malad, Dept Pediat, F-75015 Paris, France

[4] Hop Necker Enfants Malad, Dept Genet, F-75015 Paris, France

[5] Hop Necker Enfants Malad, AP HP, Lab Biochem A, F-75015 Paris, France

来源：

CLINICAL BIOCHEMISTRY | 2009年 / 42卷 / 1-2期

关键词：

Cystic fibrosis; Glutathione S-transferase; Oxidative stress; Lung infection; GENETIC POLYMORPHISMS; OXIDATIVE STRESS; P1; POLYMORPHISMS; ENZYME-ACTIVITY; DISEASE; GSTM1; SUSCEPTIBILITY; GENOTYPE; M1; T1;

D O I：

10.1016/j.clinbiochem.2008.09.116

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objectives: In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection. Methods: GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC. Results: No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%). Conclusion: GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：57 / 63

页数：7

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