Neopterin negatively regulates expression of ABCA1 and ABCG1 by the LXRα signaling pathway in THP-1 macrophage-derived foam cells

被引：14

作者：

Yan, Jin-quan ^{[1
]}

Tan, Chun-zhi ^{[2
,3
]}

Wu, Jin-hua ^{[4
]}

Zhang, Dong-cui ^{[5
]}

Chen, Ji-ling ^{[6
]}

Zeng, Bin-yuan ^{[2
]}

Jiang, Yu-ping ^{[2
]}

Nie, Jin ^{[2
]}

Liu, Wei ^{[2
]}

Liu, Qin ^{[2
]}

Dai, Hao ^{[2
]}

机构：

[1] Shaoyang Med Coll, Affiliated Hosp, Shaoyang 422000, Hunan, Peoples R China

[2] First Peoples Hosp Shaoyang City, Shaoyang 422000, Hunan, Peoples R China

[3] Univ South China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Hunan, Peoples R China

[4] Nanhua Univ, Affiliated Hosp 2, Hengyang 421001, Hunan, Peoples R China

[5] Hunan Prov Geriatr Hosp, Changsha 410000, Hunan, Peoples R China

[6] 3 Hosp Wuhan, Wuhan 430000, Hubei, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2013年 / 379卷 / 1-2期

关键词：

Neopterin; ABCA1; Cholesterol efflux; LXR alpha; Atherosclerosis; CASSETTE TRANSPORTER A1; SYSTEMIC-LUPUS-ERYTHEMATOSUS; FATTY-ACIDS REPRESS; CHOLESTEROL EFFLUX; DENSITY-LIPOPROTEIN; CORONARY; ACTIVATION; ATHEROSCLEROSIS; RISK; INFLAMMATION;

D O I：

10.1007/s11010-013-1634-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate the effects of neopterin on ABCA1 expression and cholesterol efflux in human THP-1 macrophage-derived foam cells, and to explore the role of the liver X receptor alpha (LXR alpha) involved. In the present study, THP-1 cells were pre-incubated with ox-LDL to become foam cells. The protein and mRNA expression were examined by Western blot assays and real-time quantitative PCR, respectively. Liquid scintillation counting and high performance liquid chromatography assays were used to test cellular cholesterol efflux and cholesterol content. Neopterin decreased ABCA1 expression and cholesterol efflux in a time- and concentration-dependent manner in THP-1 macrophage-derived foam cells, and the LXR alpha siRNA can reverse the inhibitory effects induced by neopterin. Neoterin has a negative regulation on ABCA1 expression via the LXR alpha signaling pathway, which suggests the aggravated effects of neopterin on atherosclerosis.

引用

页码：123 / 131

页数：9

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