Size characterization of inclusion bodies by sedimentation field-flow fractionation

被引：39

作者：

Margreiter, Gerd ^{[1
]}

Messner, Paul ^{[2
]}

Caldwell, Karin D. ^{[3
]}

Bayer, Karl ^{[1
]}

机构：

[1] Univ Nat Resources & Appl Life Sci BOKU, Dept Biotechnol, A-1190 Vienna, Austria

[2] Univ Nat Resources & Appl Life Sci BOKU, Ctr NanoBiotechnol, A-1180 Vienna, Austria

[3] Uppsala Univ, Sweden Dept Phys & Analyt Chem Surface Biotechnol, BMC, SE-75123 Uppsala, Sweden

来源：

JOURNAL OF BIOTECHNOLOGY | 2008年 / 138卷 / 3-4期

基金：

奥地利科学基金会;

关键词：

Sedimentation field-flow fractionation; Inclusion bodies; beta-Lactamase; Fed-batch cultivation;

D O I：

10.1016/j.jbiotec.2008.07.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sedimentation field-flow fractionation (sedFFF) was evaluated to characterize the size of Delta(4-23)TEM-beta-lactamase inclusion bodies (IBs) overexpressed in fed-batch cultivations of Escherichia coli. Heterologous Delta(4-23)TEM-beta-lactamase protein formed different sizes of IBs, depending upon the induction conditions. In the early phases of recombinant protein expression, induced with low concentrations of IPTG (isopropyl-beta-D-thiogalactoside), 113 masses were larger than expected and showed heterogeneous size distributions. During cultivation, IB sizes showed a Gaussian distribution and reached a broad range by the end of the fed-batch cultivations. The obtained result proved the aptitude of sedFFF to rapidly assess the size distribution of IBs in a culture. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：67 / 73

页数：7

共 25 条

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