Comparison of the immunoreactivities of NMDA receptors between the young and adult hippocampal CA1 region induced by experimentally transient cerebral ischemia

被引：6

作者：

Seo, Jeong Yeol ^{[1
]}

Yan, Bing Chun ^{[2
]}

Park, Joon Ha ^{[2
]}

Ahn, Ji Hyeon ^{[3
]}

Kim, In Hye ^{[2
]}

Lee, Jae-Chul ^{[2
]}

Kwon, Young-Geun ^{[4
]}

Kim, Young-Myeong ^{[5
,6
]}

Cho, Jun Hwi ^{[7
,8
]}

Won, Moo-Ho ^{[2
,8
]}

机构：

[1] Hallym Univ, Chuncheon Sacred Heart Hosp, Coll Med, Dept Emergency Med, Chunchon 200702, South Korea

[2] Kangwon Natl Univ, Sch Med, Dept Neurobiol, Chunchon 200701, South Korea

[3] Daegu Univ, Coll Rehabil Sci, Dept Phys Therapy, Neurosci Lab, Gyongsan 712714, South Korea

[4] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 120749, South Korea

[5] Kangwon Natl Univ, Sch Med, Vasc Syst Res Ctr, Chunchon 200701, South Korea

[6] Kangwon Natl Univ, Sch Med, Dept Mol & Cellular Biochem, Chunchon 200701, South Korea

[7] Kangwon Natl Univ, Kangwon Natl Univ Hosp, Sch Med, Dept Emergency Med, Chunchon 200701, South Korea

[8] Kangwon Natl Univ, Kangwon Natl Univ Hosp, Sch Med, Inst Med Sci, Chunchon 200701, South Korea

来源：

JOURNAL OF THE NEUROLOGICAL SCIENCES | 2013年 / 325卷 / 1-2期

基金：

新加坡国家研究基金会;

关键词：

Young animal; Ischemia-reperfusion; Pyramidal cells; Delayed neuronal death; Excitotoxicity; Gerbil; DELAYED NEURONAL DEATH; CELL-DEATH; GERBIL HIPPOCAMPUS; BRAIN ISCHEMIA; MOLECULAR-MECHANISMS; IN-VITRO; CALCIUM; EXCITOTOXICITY; GLUTAMATE; MICRODIALYSIS;

D O I：

10.1016/j.jns.2012.12.012

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Young gerbils are much more resistant to transient cerebral ischemia than the adult. In the present study, we observed that about 90% of CA1 pyramidal cells in the adult hippocampus died 4 days post-ischemia; however, about 56% of them in the young hippocampus died at 7 days post-ischemia. To compare excitotoxicity between them, we carried out immunoreactivities of NMDA receptor 1 (NMDAR1) and NMDAR2A/B in the hippocampal CA1 region (CM) induced by 5 min of transient cerebral ischemia in the young and adult gerbils. Their immunoreactivities and protein levels in the young sham-group were much lower than those in the adult sham-group. Four days after ischemia-reperfusion, they were significantly decreased in the adult ischemia-group; however, in the young ischemia-group, they were much higher than those in the adult. Seven days after ischemia-reperfusion, NMDAR1 immunoreactivity and its level in the young were much higher than those in the adult; NMDAR2A/B immunoreactivity and its level in the young were lower than in the adult. In brief, the immunoreactivities of NMDARs were not decreased in the ischemic CM region of the young 4 days after transient cerebral ischemia. This finding indicates that longer maintenance of NMDARs may contribute to less and more delayed neuronal death/damage in the young CA1. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：108 / 114

页数：7

共 30 条

[1] ISCHEMIC DELAYED NEURONAL DEATH - A MITOCHONDRIAL HYPOTHESIS [J].

ABE, K ;

AOKI, M ;

KAWAGOE, J ;

YOSHIDA, T ;

HATTORI, A ;

KOGURE, K ;

ITOYAMA, Y .

STROKE, 1995, 26 (08) :1478-1489

[2] Combined mechanical trauma and metabolic impairment in vitro induces NMDA receptor-dependent neuronal cell death and caspase-3-dependent apoptosis [J].

Allen, JW ;

Knoblach, SM ;

Faden, AI .

FASEB JOURNAL, 1999, 13 (13) :1875-1882

[3] Calpains and Delayed Calcium Deregulation in Excitotoxicity [J].

Araujo, Ines M. ;

Carreira, Bruno P. ;

Carvalho, Caetana M. ;

Carvalho, Arselio P. .

NEUROCHEMICAL RESEARCH, 2010, 35 (12) :1966-1969

[4] Molecular mechanisms of calcium-dependent neurodegeneration in excitotoxicity [J].

Arundine, M ;

Tymianski, M .

CELL CALCIUM, 2003, 34 (4-5) :325-337

[5] Transient brain ischemia: NMDA receptor modulation and delayed neuronal death [J].

Benquet, Pascal ;

Gee, Christine E. ;

Gerber, Urs .

M S-MEDECINE SCIENCES, 2008, 24 (02) :185-190

[6] ELEVATION OF THE EXTRACELLULAR CONCENTRATIONS OF GLUTAMATE AND ASPARTATE IN RAT HIPPOCAMPUS DURING TRANSIENT CEREBRAL-ISCHEMIA MONITORED BY INTRACEREBRAL MICRODIALYSIS [J].

BENVENISTE, H ;

DREJER, J ;

SCHOUSBOE, A ;

DIEMER, NH .

JOURNAL OF NEUROCHEMISTRY, 1984, 43 (05) :1369-1374

[7] Delayed treatment with nimesulide reduces measures of oxidative stress following global ischemic brain injury in gerbils [J].

Candelario-Jalil, E ;

Alvarez, D ;

Merino, N ;

León, OS .

NEUROSCIENCE RESEARCH, 2003, 47 (02) :245-253

[8] A new look at glutamate and ischemia: NMDA agonist improves long-term functional outcome in a rat model of stroke [J].

Dhawan, Jasbeer ;

Benveniste, Helene ;

Luo, Zhongchi ;

Nawrocky, Marta ;

Smith, David ;

Biegon, Anat .

FUTURE NEUROLOGY, 2011, 6 (06) :823-834

[9] MEK inhibition exacerbates ischemic calcium imbalance and neuronal cell death in rat cortical cultures [J].

Franceschini, Davide ;

Giusti, Pietro ;

Skaper, Stephen D. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 553 (1-3) :18-27

[10]

Hou ST, 2002, INT REV CYTOL, V221, P93

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