A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers

被引：31

作者：

Berenyi, Szilvia ^{[1
]}

Mihaly, Judith ^{[1
]}

Wacha, Andras ^{[1
]}

Toke, Orsolya ^{[2
]}

Bota, Attila ^{[1
]}

机构：

[1] Hungarian Acad Sci, Inst Mol Pharmacol, Res Ctr Nat Sci, Dept Biol Nanochem, H-1117 Budapest, Hungary

[2] Hungarian Acad Sci, Inst Organ Chem, Res Ctr Nat Sci, Dept Struct Chem, H-1117 Budapest, Hungary

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2014年 / 118卷

基金：

匈牙利科学研究基金会;

关键词：

Dendrimer; DPPC; Model membrane; Infrared spectroscopy; SAXS; POLY(AMIDOAMINE) DENDRIMERS; MODEL MEMBRANES; DRUG-DELIVERY; DPPC BILAYERS; CYTOTOXICITY; SPECTROSCOPY; DIFFRACTION; LIPOSOMES; DETECTOR; PILATUS;

D O I：

10.1016/j.colsurfb.2014.03.048

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The effect of 5th generation polyamidoamine (PAMAM G5) dendrimers on multilamellar dipalmi-toylphosphocholine (DPPC) vesicles was investigated. PAMAM was added in two different concentrations to the lipids (10(-3) and 10(-2) dendrimer/lipid molar ratios). The thermal behavior of the evolved systems was characterized by DSC; while the structure and the morphology were investigated with small- and wide-angel X-ray scattering (SWAXS), freeze-fracture electron microscopy (FFTEM) and phosphorus-31 nuclear magnetic resonance (P-31 NMR) spectroscopy, respectively. IR spectroscopy was used to study the molecular interactions between PAMAM and DPPC. The obtained results show that the dendrimers added in 10(-3) molar ratio to the lipids generate minor perturbations in the multilamellar structure and thermal character of liposomes, while added in 10(-2) molar ratio dendrimers cause major disturbance in the vesicular system. The terminal amino groups of the dendrimers are in strong interaction with the phosphate headgroups and through this binding dendrimers disrupt the regular multilamellar structure of DPPC. Besides highly swollen, fragmented bilayers, small vesicles are formed. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：164 / 171

页数：8

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