Isolation and characterization of 13 microsatellite markers for the viviparous surfperch Ditrema temmincki (Embiotocidae) and cross-species amplification

被引：3

作者：

Takagi, Motohiro ^{[1
]}

Sakai, Kumiko ^{[1
]}

Taniguchi, Nobuhiko ^{[2
]}

机构：

[1] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan

[2] Fukuyama Univ, Res Inst Marine Bioresources, Innoshima, Onomichi 7222101, Japan

来源：

MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 05期

关键词：

cross-amplification; Ditrema; microsatellite; viviparous surfperch;

D O I：

10.1111/j.1755-0998.2008.02145.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thirteen microsatellite loci were isolated from a size-selected genomic library of the surfperch (Ditrema temmincki Bleeker). All loci displayed a high degree of length polymorphism, as observed in the total number of alleles per locus (two to 23) and a high degree of estimated heterozygosity ranging from 0.080 to 0.893. The primers developed for D. temmincki were also tested for their ability to amplify homologous sequences in D. viride and Neoditrema ransonetii. Distinct differences were observed among three species of surfperches, in both genetic variability and the frequency distribution of the alleles.

引用

页码：1030 / 1033

页数：4

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