In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

被引：37

作者：

Tsai, Tien-Chun ^{[1
]}

Liu, Chia-Wei ^{[1
,2
]}

Wu, Yi-Chen ^{[1
]}

Ondevilla, Neil Adrian P. ^{[1
]}

Osawa, Masatoshi ^{[3
]}

Chang, Hsien-Chang ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Biomed Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 70101, Taiwan

[3] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0640806, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 175卷

关键词：

Surface-enhanced infrared absorption spectroscopy (SEIRAS); Attenuated total reflection (ATR); EDC/NHS; ELECTROCHEMICAL IMMUNOSENSOR; ELECTROLESS DEPOSITION; ADSORBED PHOSPHATE; MONOLAYERS; MALEIMIDE; BIOSENSOR;

D O I：

10.1016/j.colsurfb.2018.12.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

引用

页码：300 / 305

页数：6

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