In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

被引：37

作者：

Tsai, Tien-Chun ^{[1
]}

Liu, Chia-Wei ^{[1
,2
]}

Wu, Yi-Chen ^{[1
]}

Ondevilla, Neil Adrian P. ^{[1
]}

Osawa, Masatoshi ^{[3
]}

Chang, Hsien-Chang ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Biomed Engn, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 70101, Taiwan

[3] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0640806, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 175卷

关键词：

Surface-enhanced infrared absorption spectroscopy (SEIRAS); Attenuated total reflection (ATR); EDC/NHS; ELECTROCHEMICAL IMMUNOSENSOR; ELECTROLESS DEPOSITION; ADSORBED PHOSPHATE; MONOLAYERS; MALEIMIDE; BIOSENSOR;

D O I：

10.1016/j.colsurfb.2018.12.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

引用

页码：300 / 305

页数：6

共 5 条

[1] Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology
Seica, Ana F. S.
Iqbal, Muhammad Haseeb
Carvalho, Alain
Choe, Jun-yong
Boulmedais, Fouzia
Hellwig, Petra
ACS SENSORS, 2021, 6 (08) : 2875 - 2882
[2] Surface-enhanced infrared absorption spectroscopy (SEIRAS) for biochemical analysis: Progress and perspective
Wang, Jikai
Xie, Zhulan
Zhu, Yanli
Zeng, Pengfei
He, Suisui
Wang, Jun
Wei, Hua
Yu, Cuiyun
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY, 2024, 41
[3] Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy
Liu, Chia-Wei
Tsai, Tien-Chun
Osawa, Masatoshi
Chang, Hsien-Chang
Yang, Ruey-Jen
ANALYTICA CHIMICA ACTA, 2018, 1033 : 137 - 147
[4] Hybrid Gold-Conductive Metal Oxide Films for Attenuated Total Reflectance Surface Enhanced Infrared Absorption Spectroscopy
Andvaag, Ian R.
Morhart, Tyler A.
Clarke, Osai J. R.
Burgess, Ian J.
ACS APPLIED NANO MATERIALS, 2019, 2 (03) : 1274 - 1284
[5] Distortion of the amide-I and -II bands of an α-helical membrane protein, pharaonis halorhodopsin, depends on thickness of gold films utilized for surface-enhanced infrared absorption spectroscopy
Guo, Hao
Kimura, Tetsunari
Furutani, Yuji
CHEMICAL PHYSICS, 2013, 419 : 8 - 16

← 1 →