In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

被引：43
作者：
Tsai, Tien-Chun [1 ]
Liu, Chia-Wei [1 ,2 ]
Wu, Yi-Chen [1 ]
Ondevilla, Neil Adrian P. [1 ]
Osawa, Masatoshi [3 ]
Chang, Hsien-Chang [1 ]
机构：
[1] Natl Cheng Kung Univ, Dept Biomed Engn, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 70101, Taiwan
[3] Hokkaido Univ, Catalysis Res Ctr, Sapporo, Hokkaido 0640806, Japan
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2019年 / 175卷
关键词：
Surface-enhanced infrared absorption spectroscopy (SEIRAS); Attenuated total reflection (ATR); EDC/NHS; ELECTROCHEMICAL IMMUNOSENSOR; ELECTROLESS DEPOSITION; ADSORBED PHOSPHATE; MONOLAYERS; MALEIMIDE; BIOSENSOR;
D O I：
10.1016/j.colsurfb.2018.12.009
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).
引用
收藏
页码：300 / 305
页数：6
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