Development of bioactive and biodegradable chitosan-based injectable systems containing bioactive glass nanoparticles

被引：118
作者：
Couto, Daniela S. [1 ,2 ]
Hong, Zhongkui [1 ,2 ]
Mano, Joao F. [1 ,2 ]
机构：
[1] Dept Polymer Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, P-4710057 Braga, Portugal
[2] Inst Biotechnol & Bioengn, IBB, Braga, Portugal
来源：
ACTA BIOMATERIALIA | 2009年 / 5卷 / 01期
关键词：
Chitosan; Inorganic nanoparticles; Hydrogels; Biomineralization; Stimuli-responsive; HYDROGELS; MEDICINE; GELATION; BIOLOGY; CHITIN;
D O I：
10.1016/j.actbio.2008.08.006
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
There is increasing interest in the development of new tissue engineering strategies to deliver cells and bioactive agents encapsulated in a biodegradable matrix through minimally invasive procedures. The present work proposes to combine chitosan-beta-glycerophosphate salt formulations with bioactive glass nanoparticles in order to conceive novel injectable thermo-responsive hydrogels for orthopaedic, reconstructive and regenerative medicine applications. The initial theological properties and the gelation points of the developed organic-inorganic in situ thermosetting systems were revealed to be adequate for intracorporal injection. In vitro bioactivity tests, using incubation protocols in simulated body fluid (SBF), allowed the observation of bone-like apatite formation in the hydrogel formulations containing bioactive nanoparticles. The density of the apatite formed increased with increasing bioactive glass content and soaking time in SBF. These results indicate that the stimuli-responsive hydrogels could potentially be used as temporary injectable scaffolds in bone tissue engineering applications. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：115 / 123
页数：9
相关论文
共 33 条
[1]  
Ballard JD, 2006, WILEY ENCY BIOMEDICA, V6
[2]   Rheological characterisation of thermogelling chitosan/glycerol-phosphate solutions [J].
Chenite, A ;
Buschmann, M ;
Wang, D ;
Chaput, C ;
Kandani, N .
CARBOHYDRATE POLYMERS, 2001, 46 (01) :39-47
[3]   Novel injectable neutral solutions of chitosan form biodegradable gels in situ [J].
Chenite, A ;
Chaput, C ;
Wang, D ;
Combes, C ;
Buschmann, MD ;
Hoemann, CD ;
Leroux, JC ;
Atkinson, BL ;
Binette, F ;
Selmani, A .
BIOMATERIALS, 2000, 21 (21) :2155-2161
[4]   Physical gelation of chitosan in the presence of β-glycerophosphate:: The effect of temperature [J].
Cho, JY ;
Heuzey, MC ;
Bégin, A ;
Carreau, PJ .
BIOMACROMOLECULES, 2005, 6 (06) :3267-3275
[5]   Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry [J].
da Silva, Ricardo M. P. ;
Mano, Jado F. ;
Reis, Rui L. .
MACROMOLECULAR CHEMISTRY AND PHYSICS, 2008, 209 (14) :1463-1472
[6]   Gelation time and degradation rate of chitosan-based injectable hydrogel [J].
Ganji, F. ;
Abdekhodaie, M. J. ;
Ramazani S. A., A. .
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2007, 42 (01) :47-53
[7]   Chitosan microparticles as injectable scaffolds for tissue engineering [J].
Garcia Cruz, Dunia Mercedes ;
Escobar Ivirico, Jorge Luis ;
Gomes, Manuela M. ;
Gomez Ribelles, Jose Luis ;
Salmeron Sanchez, Manuel ;
Reis, Rui L. ;
Mano, Joao F. .
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2008, 2 (06) :378-380
[8]  
HENCH L L, 1972, Journal of Biomedical Materials Research Biomedical Materials Symposium, V2, P117
[9]   Hydrogels for biomedical applications [J].
Hoffman, Allan S. .
ADVANCED DRUG DELIVERY REVIEWS, 2012, 64 :18-23
[10]  
HONG Z, J BIOMED A IN PRESS
1234