Release kinetics of circulating miRNA-208a in the early phase of myocardial infarction

被引：41

作者：

Bialek, Slawomir ^{[1
]}

Gorko, Dariusz ^{[2
]}

Zajkowska, Agnieszka ^{[3
]}

Koltowski, Lukasz ^{[2
]}

Grabowski, Marcin ^{[2
]}

Stachurska, Anna ^{[1
]}

Kochman, Janusz ^{[2
]}

Sygitowicz, Grazyna ^{[1
]}

Malecki, Maciej ^{[3
]}

Opolski, Grzegorz ^{[2
]}

Sitkiewicz, Dariusz ^{[1
]}

机构：

[1] Med Univ Warsaw, Div Lab Med, Fac Pharm, Dept Med Lab Diagnost, PL-02097 Warsaw, Poland

[2] Med Univ Warsaw, Fac Med 1, Dept Cardiol 1, PL-02097 Warsaw, Poland

[3] Med Univ Warsaw, Div Lab Med, Fac Pharm, Dept Mol Biol, PL-02097 Warsaw, Poland

来源：

KARDIOLOGIA POLSKA | 2015年 / 73卷 / 08期

关键词：

miRNA-208a; myocardial infarction; cardiac markers; SENSITIVE BIOMARKERS; CARDIAC TROPONIN; MICRORNAS; DIAGNOSIS;

D O I：

10.5603/KP.a2015.0067

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: The biochemical confirmation of myocardial infarction is based on cardiac troponin (cTnI or cTnT) determination. Recent scientific results suggested that microRNAs (miRNAs) might become a new biomarker of tissue injury. Aim: To evaluate the release kinetics of circulating heart-specific miRNA-208a and also to test the hypothesis that miRNA-208a can serve as an accessible, diagnostically sensitive plasma biomarker of ST-elevation acute myocardial infarction (STEMI). Methods: Nineteen STEMI patients (four women and 15 men, aged 44-85 years), 12 patients with stable coronary artery disease (CAD), and eight patients with a negative observation of CAD as a control group were studied. Blood samples were collected on admission and at three, six, 12, 24, and 48 h afterwards; in the CAD and control group blood samples were taken only once. Plasma levels of miRNA-208a determined by real-time polymerase chain reaction and their relative fold changes were calculated. cTnI and creatinine kinase (CK)-MB mass were also measured in the patients' serum samples. Results: miRNA-208a was increased in STEMI patients at the time of admission and nearly undetectable in CAD patients and controls. The peak of miRNA-208a was observed at 3 h after reperfusion (p < 0.001). The traditional biomarkers (cTnI and CK-MBmass), which increase later in comparison to miRNA-208a reaching the maximum concentrations 6 h after reperfusion, were observed. Circulating miRNA-208a levels strongly correlated with cTnI and CK-MBmass released from the infarcted area. Conclusions: These results demonstrate that plasma miRNA-208a is an interesting and promising candidate for a new biomarker released early after onset of myocardial infarction.

引用

页码：613 / 619

页数：7

共 28 条

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