Decolorization of 1:2 metal complex dye Acid blue 193 by a newly isolated fungus, Cladosporium cladosporioides

被引:29
作者
Vijaykumar, MH [1 ]
Veeranagouda, Y [1 ]
Neelakanteshwar, K [1 ]
Karegoudar, TB [1 ]
机构
[1] Gulbarga Univ, Dept Biochem, Gulbarga 585106, Karnataka, India
关键词
Cladosporium cladosporioides; dye decolorization; laccase; manganese peroxidase; metabolites;
D O I
10.1007/s11274-005-9013-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A fungus Cladosporium cladosporioides isolated from coal sample as a decolorizing microorganism. It decolorized five different azo and triphenylmethane dyes like acid blue 193, acid black 210, crystal violet, reactive black B(S) and reactive black BL/LPR both on solid and in liquid broth medium. Culture broth of this fungus decolorized completely 100 mg of acid blue 193 l(-1) in 8 days. The extracellular enzyme of Cladosporium cladosporioides decolorized acid blue 193 on repeated addition to a total (out of 700 mg l(-1)) concentration of 564 mg l(-1) within 168 h without significant decline in the activity, showing the resistant property of Cladosporium cladosporioides to a high concentration of the dye. The optimal temperature 40 degrees C, pH 5.6 and sugar concentration of 4% required for decolorization of acid blue 193. Cladosporium cladosporioides showed manganese peroxidase activity with 41 U l(-1), laccase activity with 1413 U l(-1) and lignin peroxidase activity was negligible after day 8 of incubation.
引用
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页码:157 / 162
页数:6
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