Translocation of SiO2-NPs across in vitro human bronchial epithelial monolayer

被引：3

作者：

George, I. ^{[1
]}

Vranic, S. ^{[1
]}

Boland, S. ^{[1
]}

Borot, M. C. ^{[1
]}

Marano, F. ^{[1
]}

Baeza-Squiban, A. ^{[1
]}

机构：

[1] Univ Paris Diderot, Lab Reponses Mol & Cellulaires Xenobiot, Unite Biol Fonct & Adaptat, Sorbonne Paris Cite,EAC CNRS 4413, F-75205 Paris 13, France

来源：

NANOSAFE 2012: INTERNATIONAL CONFERENCES ON SAFE PRODUCTION AND USE OF NANOMATERIALS | 2013年 / 429卷

关键词：

INHALED NANOPARTICLES; PARTICLES; LUNG;

D O I：

10.1088/1742-6596/429/1/012022

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Safe development and application of nanotechnologies in many fields require better knowledge about their potential adverse effects on human health. Evidence of abilities of nanoparticles (NPs) to cross epithelial barriers and reach secondary organs via the bloodstream led us to investigate the translocation of SiO2 NPs of 50 nm (50 nm-SiO2-NPs) across human bronchial epithelial cells that are primary targets after exposure to inhaled NPs. We quantified the translocation of fluorescently labelled SiO2 NPs at non-cytotoxic concentrations (5 and 10 mu g/cm(2)) across Calu-3 epithelial monolayer. After 14 days in culture Calu-3 cells seeded onto 3 mu m-polycarbonate Transwell membranes formed an efficient bronchial barrier assessed by measurement of the transepithelial electric resistance and quantification of the permeability of the monolayer. After 24 hours of exposure, we observed a significant translocation of NPs that was more important when the initial NP concentration decreased. Confocal microscopy observations revealed NP uptake by cells and an important NP retention inside the porous membrane. In conclusion, 50 nm-SiO2-NPs can cross the human bronchial epithelial barrier without affecting the integrity of the epithelial cell monolayer.

引用

页数：10

共 14 条

[1] Human lung tissue engineering: a critical tool for safer medicines [J].