Effects of microwave on extracellular vesicles and microRNA in milk

被引：30

作者：

Zhao, Zhehao ^{[1
]}

Yu, Siran ^{[1
]}

Xu, Minjing ^{[1
]}

Li, Ping ^{[1
]}

机构：

[1] Tongji Univ, Shanghai East Hosp, Res Ctr Translat Med, Sch Life Sci & Technol, Shanghai 200092, Peoples R China

来源：

JOURNAL OF DAIRY SCIENCE | 2018年 / 101卷 / 04期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

milk RNA; microRNA; extracellular vesicles; microwave; HEPATOCELLULAR-CARCINOMA; PERIPHERAL-BLOOD; PROSTATE-CANCER; BODY-FLUIDS; SMALL RNAS; COW MILK; CELLS; MORTALITY; PRODUCTS; EXOSOMES;

D O I：

10.3168/jds.2016-12021

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study demonstrates the effects of microwaves on the microRNA (miRNA) content of milk and milk extracellular vesicles (EV). We determined the miRNA concentration in milk subjected to different treatments using real-time PCR and a spectrophotometer. The miRNA expression and total RNA content of the microwaved milk samples were lower when compared with untreated milk. We measured the microstructure and the size distribution by scanning electron microscopy and dynamic light scattering to verify the loss of miRNA in microwaved milk due to damage to the EV. The results revealed that 2 different-sized EV were present and had an average size of 147.50 and 22.14 nm, respectively. Furthermore, acridine orange staining showed that the total RNA content in microwaved milk EV was lower than that in cow milk. These results suggest that EV may confer the protection and the stability of the miRNA in milk.

引用

页码：2932 / 2940

页数：9

共 40 条

[1] Physicochemical Biomolecular Insights into Buffalo Milk-Derived Nanovesicles [J].

Baddela, Vijay Simha ;

Nayan, Varij ;

Rani, Payal ;

Onteru, Suneel Kumar ;

Singh, Dheer .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2016, 178 (03) :544-557

[2] MicroRNAs Are Absorbed in Biologically Meaningful Amounts from Nutritionally Relevant Doses of Cow Milk and Affect Gene Expression in Peripheral Blood Mononuclear Cells, HEK-293 Kidney Cell Cultures, and Mouse Livers [J].

Baier, Scott R. ;

Nguyen, Christopher ;

Xie, Fang ;

Wood, Jennifer R. ;

Zempleni, Janos .

JOURNAL OF NUTRITION, 2014, 144 (10) :1495-1500

[3] MicroRNA-34a regulates cardiac ageing and function [J].

Boon, Reinier A. ;

Iekushi, Kazuma ;

Lechner, Stefanie ;

Seeger, Timon ;

Fischer, Ariane ;

Heydt, Susanne ;

Kaluza, David ;

Treguer, Karine ;

Carmona, Guillaume ;

Bonauer, Angelika ;

Horrevoets, Anton J. G. ;

Didier, Nathalie ;

Girmatsion, Zenawit ;

Biliczki, Peter ;

Ehrlich, Joachim R. ;

Katus, Hugo A. ;

Mueller, Oliver J. ;

Potente, Michael ;

Zeiher, Andreas M. ;

Hermeking, Heiko ;

Dimmeler, Stefanie .

NATURE, 2013, 495 (7439) :107-110

[4] MicroRNA signatures in human cancers [J].

Calin, George A. ;

Croce, Carlo M. .

NATURE REVIEWS CANCER, 2006, 6 (11) :857-866

[5] Microwave food processing-A review [J].

Chandrasekaran, S. ;

Ramanathan, S. ;

Basak, Tanmay .

FOOD RESEARCH INTERNATIONAL, 2013, 52 (01) :243-261

[6] Exploration of microRNAs in porcine milk exosomes [J].

Chen, Ting ;

Xi, Qian-Yun ;

Ye, Rui-Song ;

Cheng, Xiao ;

Qi, Qi-En ;

Wang, Song-Bo ;

Shu, Gang ;

Wang, Li-Na ;

Zhu, Xiao-Tong ;

Jiang, Qing-Yan ;

Zhang, Yong-Liang .

BMC GENOMICS, 2014, 15

[7] Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products [J].

Chen, Xi ;

Gao, Chao ;

Li, Haijin ;

Huang, Lei ;

Sun, Qi ;

Dong, Yanye ;

Tian, Chunliang ;

Gao, Shengpu ;

Dong, Hailin ;

Guan, Danping ;

Hu, Xiaoyun ;

Zhao, Shujian ;

Li, Liang ;

Zhu, Lin ;

Tan, Qiao ;

Zhang, Junfeng ;

Zen, Ke ;

Zhang, Chen-Yu .

CELL RESEARCH, 2010, 20 (10) :1128-1137

[8]

Darzynkiewicz Z, 1990, Methods Cell Biol, V33, P285

[9] The Effect of Microwave Pasteurization on Some Physical and Chemical Characteristics of Milk [J].

Dehghan, Afsaneh ;

Jamalian, Jalal ;

Farahnaky, Asgar ;

Mesbahi, Gholamreza ;

Moosavi-Nasab, Marzieh .

INTERNATIONAL JOURNAL OF FOOD ENGINEERING, 2012, 8 (01)

[10] Dairy products and risk of hepatocellular carcinoma: The European Prospective Investigation into Cancer and Nutrition [J].

Duarte-Salles, Talita ;

Fedirko, Veronika ;

Stepien, Magdalena ;

Trichopoulou, Antonia ;

Bamia, Christina ;

Lagiou, Pagona ;

Lukanova, Annekatrin ;

Trepo, Elisabeth ;

Overvad, Kim ;

Tjonneland, Anne ;

Halkjaer, Jytte ;

Boutron-Ruault, Marie-Christine ;

Racine, Antoine ;

Cadeau, Claire ;

Kuehn, Tilman ;

Aleksandrova, Krasimira ;

Trichopoulos, Dimitrios ;

Tsiotas, Konstantinos ;

Boffetta, Paolo ;

Palli, Domenico ;

Pala, Valeria ;

Tumino, Rosario ;

Sacerdote, Carlotta ;

Panico, Salvatore ;

Bueno-de-Mesquita, H. B ;

Dik, Vincent K. ;

Peeters, Petra H. ;

Weiderpass, Elisabete ;

Gram, Inger Torhild ;

Hjartaker, Anette ;

Ramon Quiros, Jose ;

Fonseca-Nunes, Ana ;

Molina-Montes, Esther ;

Dorronsoro, Miren ;

Navarro Sanchez, Carmen ;

Barricarte, Aurelio ;

Lindkvist, Bjorn ;

Sonestedt, Emily ;

Johansson, Ingegerd ;

Wennberg, Maria ;

Khaw, Kay-Tee ;

Wareham, Nick ;

Travis, Ruth C. ;

Romieu, Isabelle ;

Riboli, Elio ;

Jenab, Mazda .

INTERNATIONAL JOURNAL OF CANCER, 2014, 135 (07) :1662-1672

← 1 2 3 4 →