Overexpression of O-methyltransferase leads to improved vanillin production in baker's yeast only when complemented with model-guided network engineering

被引：35

作者：

Brochado, Ana Rita ^{[1
,2
]}

Patil, Kiran R. ^{[2
]}

机构：

[1] Tech Univ Denmark, Ctr Microbial Biotechnol, Dept Syst Biol, DK-2800 Lyngby, Denmark

[2] EMBL Heidelberg, Struct & Computat Biol Unit, European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2013年 / 110卷 / 02期

关键词：

vanillin; Saccharomyces cerevisiae; pathway engineering; ESCHERICHIA-COLI; CLONING; ACID;

D O I：

10.1002/bit.24731

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Overproduction of a desired metabolite is often achieved via manipulation of the pathway directly leading to the product or through engineering of distant nodes within the metabolic network. Empirical examples illustrating the combined effect of these local and global strategies have been so far limited in eukaryotic systems. In this study, we compared the effects of overexpressing a key gene in de novo vanillin biosynthesis (coding for O-methyltransferase, hsOMT) in two yeast strains, with and without model-guided global network modifications. Overexpression of hsOMT resulted in increased vanillin production only in the strain with model-guided modifications, exemplifying advantage of using a global strategy prior to local pathway manipulation. Biotechnol. Bioeng. 2013; 110: 656659. (c) 2012 Wiley Periodicals, Inc.

引用

页码：656 / 659

页数：4

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