Methyl group balance in brain and liver: Role of choline on increased S-adenosyl methionine (SAM) demand by chronic arsenic exposure

被引：16

作者：

Rios, Rosalva ^{[1
]}

Santoyo, Martha E. ^{[1
]}

Cruz, Daniela ^{[1
]}

Manuel Delgado, Juan ^{[1
]}

Zarazua, Sergio ^{[2
]}

Jimenez-Capdeville, Maria E. ^{[1
]}

机构：

[1] Univ Autonoma San Luis Potosi, Fac Med, Dept Bioquim, San Luis Potosi Slp 78210, Mexico

[2] Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Dept Toxicol, San Luis Potosi Slp 78210, Mexico

来源：

TOXICOLOGY LETTERS | 2012年 / 215卷 / 02期

关键词：

Choline; Arsenic; Methyl donors; SAM; SODIUM ARSENITE; METABOLISM; MYELIN; CYCLOLEUCINE; TOXICITY; FOLATE; WATER; PHOSPHATIDYLCHOLINE; ADENOSYLMETHIONINE; HYPOMETHYLATION;

D O I：

10.1016/j.toxlet.2012.10.005

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Arsenic toxicity has been related to its interference with one carbon metabolism, where a high demand of S-adenosylmethionine (SAM) for arsenic methylation as well as a failure of its regeneration would compromise the availability of methyl groups for diverse cellular functions. Since exposed animals show disturbances of methylated products such as methylated arginines, myelin and axon membranes, this work investigates whether alterations of SAM, choline and phosphatidylcholine (PC) in the brain of arsenic exposed rats are associated with myelin alterations and myelin basic protein (MBP) immunoreactivity. Also these metabolites, morphologic and biochemical markers of methyl group alterations were analyzed in the liver, the main site of arsenic methylation. In adult, life-long arsenic exposed rats through drinking water (3 ppm), no changes of SAM, choline and PC concentrations where found in the brain, but SAM and PC were severely decreased in liver accompanied by a significant increase of choline. These results suggest that choline plays an important role as methyl donor in arsenic exposure, which could underlie hepatic affections observed when arsenic exposure is combined with other environmental factors. Also, important myelin and nerve fiber alterations, accompanied by a 75% decrease of MBP immunoreactivity were not associated with a SAM deficit in the brain. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：110 / 118

页数：9

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