Significant divergence in nucleotide sequences for beta-tubulin from different laboratory strains of Chinese hamster ovary cells

被引：2

作者：

Boggs, BA ^{[1
]}

GonzalezGaray, ML ^{[1
]}

Cabral, F ^{[1
]}

机构：

[1] UNIV TEXAS,SCH MED,DEPT PHARMACOL,HOUSTON,TX 77225

来源：

DNA SEQUENCE | 1996年 / 6卷 / 03期

关键词：

cDNA; CHO cells; isotype I; tubulin;

D O I：

10.3109/10425179609010205

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequences for isotype I beta-tubulin cDNAs cloned from different laboratory strains of Chinese hamster ovary (CHO) cells were compared and found to contain an unexpected number of sequence differences in both translated and untranslated regions of the gene. The results indicate significant changes in the DNA, but not protein, sequence while the cells have been in culture and reveal sequences in the 5' and 3' untranslated regions that have resisted these changes.

引用

页码：171 / 174

页数：4

共 15 条

[1] NUCLEOTIDE-SEQUENCES OF 3 DIFFERENT ISOFORMS OF BETA-TUBULIN CDNA FROM CHINESE-HAMSTER OVARY CELLS [J].

AHMAD, S ;

SINGH, B ;

GUPTA, RS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1090 (02) :252-254

[2] MUTATIONS AFFECTING ASSEMBLY AND STABILITY OF TUBULIN - EVIDENCE FOR A NONESSENTIAL BETA-TUBULIN IN CHO CELLS [J].

BOGGS, B ;

CABRAL, F .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2700-2707

[3] RESISTANCE TO ANTIMITOTIC AGENTS AS GENETIC PROBES OF MICROTUBULE STRUCTURE AND FUNCTION [J].

CABRAL, F ;

BARLOW, SB .

PHARMACOLOGY & THERAPEUTICS, 1991, 52 (02) :159-171

[4] CHO MUTANTS RESISTANT TO COLCHICINE, COLCEMID OR GRISEOFULVIN HAVE AN ALTERED BETA-TUBULIN [J].

CABRAL, F ;

SOBEL, ME ;

GOTTESMAN, MM .

CELL, 1980, 20 (01) :29-36

[5]

CLEVELAND DW, 1985, ANNU REV BIOCHEM, V54, P331, DOI 10.1146/annurev.bi.54.070185.001555

[6] GENETICS OF SOMATIC MAMMALIAN CELLS .7. INDUCTION AND ISOLATION OF NUTRITIONAL MUTANTS IN CHINESE HAMSTER CELLS [J].

KAO, FT ;

PUCK, TT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (04) :1275-+

[7]

LEE MG, 1983, CELL, V33, P477

[8] 3 EXPRESSED SEQUENCES WITHIN THE HUMAN BETA-TUBULIN MULTIGENE FAMILY EACH DEFINE A DISTINCT ISOTYPE [J].

LEWIS, SA ;

GILMARTIN, ME ;

HALL, JL ;

COWAN, NJ .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 182 (01) :11-20

[9] INVIVO MICROTUBULES ARE COPOLYMERS OF AVAILABLE BETA-TUBULIN ISOTYPES - LOCALIZATION OF EACH OF 6 VERTEBRATE BETA-TUBULIN ISOTYPES USING POLYCLONAL ANTIBODIES ELICITED BY SYNTHETIC PEPTIDE ANTIGENS [J].

LOPATA, MA ;

CLEVELAND, DW .

JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1707-1720

[10] A CDNA CLONING VECTOR THAT PERMITS EXPRESSION OF CDNA INSERTS IN MAMMALIAN-CELLS [J].

OKAYAMA, H ;

BERG, P .

MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (02) :280-289

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