Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts

被引：131

作者：

Apostolou, Anna ^{[2
]}

Stagos, Dimitrios ^{[1
]}

Galitsiou, Elissavet ^{[1
]}

Spyrou, Argiris ^{[1
]}

Haroutounian, Serko ^{[2
]}

Portesis, Nikolaos ^{[1
]}

Trizoglou, Ioanna ^{[1
]}

Hayes, A. Wallace ^{[3
,4
]}

Tsatsakis, Aristides M. ^{[5
]}

Kouretas, Dimitrios ^{[1
]}

机构：

[1] Univ Thessaly, Dept Biochem & Biotechnol, Larisa 41221, Greece

[2] Agr Univ Athens, GR-11855 Athens, Greece

[3] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA

[4] Spherix Consulting Inc, Bethesda, MD 20817 USA

[5] Univ Crete, Sch Med, Toxicol Lab, Iraklion 71003, Greece

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 61卷

关键词：

Grape stem extracts; Vitis vinifera; Polyphenols; Antioxidant; DNA damage; Anticancer activity; GRAPE SEED EXTRACT; GREEK VARIETIES; LIPID-PEROXIDATION; ARREST; CANCER; GROWTH; LEAF; WINE; PROCYANIDINS; OXIDATION;

D O I：

10.1016/j.fct.2013.01.029

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Grape extracts and wine have been studied widely due to their beneficial effects on human health. However, there are only few studies from grape stems extracts. Therefore, the main objective of the present study was the assessment in stem extracts from Greek Vitis vinifera varieties of the total polyphenolic content (TPC), the identification of the polyphenols present in them, and the evaluation of their antioxidant activity, protection against ROS-induced DNA damage and inhibition of liver (HepG2) and cervical (HeLa) cancer cell growth. The range of the TPC in grape stem extracts was from 345 to 584 mg GAE/g dry weight. Moreover, stem extracts contained different classes of polyphenols as flavonols, flavanols, procyanidins, phenolic acids and stilbenes. In DPPH and ABTS assays, the IC50 values of the stem extracts had an average of 7.8 +/- 2.8 and 5.4 +/- 2.6 mu g/mL respectively. Also, all stem extracts inhibited OH center dot- and ROO center dot- induced DNA damage dose dependent with average IC50 values of 478 +/- 217 and 1.15 +/- 0.85 mu g/mL respectively. Furthermore, stem extracts inhibited at low concentrations the growth of HepG2 and HeLa cancer cells with average IC50 values of 50 +/- 12 and 32 +/- 16 mu g/mL respectively. The above activities of grape stem extracts were comparable to those of seed extracts. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：60 / 68

页数：9

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