Peptide substrate for caspase-3 with 2-aminoacridone as reporting group

被引：8
|
作者：
Lozanov, Valentin [1 ]
Ivanov, Ivaylo P. [2 ]
Benkova, Bistra [1 ]
Mitev, Vanio [1 ]
机构：
[1] Med Univ Sofia, Dept Chem & Biochem, Sofia 1431, Bulgaria
[2] Univ Sofia St Kl Ohridsky, Fac Biol, Sofia 1164, Bulgaria
来源：
AMINO ACIDS | 2009年 / 36卷 / 03期
关键词：
2-Aminoacridone; Fluorogenic substrate; Fluorescent substrate; Caspase-3; Apoptosis; PRECOLUMN FLUORESCENCE DERIVATIZATION; APOPTOSIS; ASSAY; PROTEINASES; SPECIFICITY; ACTIVATION; MEMBERS; DESIGN; FAMILY; ENZYME;
D O I：
10.1007/s00726-008-0136-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.
引用
收藏
页码：581 / 586
页数：6
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