Nonreplicative RNA recombination in poliovirus

被引:101
作者
Gmyl, AP
Belousov, EV
Maslova, SV
Khitrina, EV
Chetverin, AB
Agol, VI
机构
[1] Moscow MV Lomonosov State Univ, Moscow 119899, Russia
[2] Russian Acad Sci, Inst Prot Res, Pushchino 142292, Russia
[3] Russian Acad Med Sci, Inst Poliomyelitis & Viral Encephalitis, Moscow 142782, Moscow Region, Russia
关键词
D O I
10.1128/JVI.73.11.8958-8965.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Current models of recombination between viral RNAs are based on replicative template-switch mechanisms. The existence of nonreplicative RNA recombination in poliovirus is demonstrated in the present study by the rescue of viable viruses after cotransfections with different pairs of genomic RNA fragments with suppressed translatable and replicating capacities. Approximately 100 distinct recombinant genomes have been identified. The majority of crossovers occurred between nonhomologous segments of the partners and might have resulted from transesterification reactions, not necessarily involving an enzymatic activity. Some of the crossover loci are clustered. The origin of some of these "hot spots" could be explained by invoking structures similar to known ribozymes, A significant proportion of recombinant RNAs contained the entire 5' partner, if its 3' end was oxidized or phosphorylated prior to being mixed with the 3' partner. All of these observations are consistent with a mechanism that involves intermediary formation of the 2',3'-cyclic phosphate and 5'-hydroxyl termini, It is proposed that nonreplicative RNA recombination may contribute to evolutionarily significant RNA rearrangements.
引用
收藏
页码:8958 / 8965
页数:8
相关论文
共 47 条
[1]  
Adams RLP, 1986, BIOCH NUCL ACIDS, P287
[2]   Recombination and other genomic rearrangements in picornaviruses [J].
Agol, VI .
SEMINARS IN VIROLOGY, 1997, 8 (02) :77-84
[3]   A FUNCTIONAL RIBONUCLEOPROTEIN COMPLEX FORMS AROUND THE 5' END OF POLIOVIRUS RNA [J].
ANDINO, R ;
RIECKHOF, GE ;
BALTIMORE, D .
CELL, 1990, 63 (02) :369-380
[4]   Viroid processing: Switch from cleavage to ligation is driven by a change from a tetraloop to a loop E conformation [J].
Baumstark, T ;
Schroder, ARW ;
Riesner, D .
EMBO JOURNAL, 1997, 16 (03) :599-610
[5]   The structure, function and application of the hammerhead ribozyme [J].
Birikh, KR ;
Heaton, PA ;
Eckstein, F .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 245 (01) :1-16
[6]  
CECH TR, 1986, ANNU REV BIOCHEM, V55, P599, DOI 10.1146/annurev.bi.55.070186.003123
[7]   Nonhomologous RNA recombination in a cell-free system: Evidence for a transesterification mechanism guided by secondary structure [J].
Chetverin, AB ;
Chetverina, HV ;
Demidenko, AA ;
Ugarov, VI .
CELL, 1997, 88 (04) :503-513
[8]   Recombination in bacteriophage Qβ and its satellite RNAs:: The in vivo and in vitro studies [J].
Chetverin, AB .
SEMINARS IN VIROLOGY, 1997, 8 (02) :121-129
[9]   Spontaneous rearrangements in RNA sequences [J].
Chetverina, HV ;
Demidenko, AA ;
Ugarov, VI ;
Chetverin, AB .
FEBS LETTERS, 1999, 450 (1-2) :89-94
[10]   Peach latent mosaic viroid is locked by a 2',5'-phosphodiester bond produced by in vitro self-ligation [J].
Cote, F ;
Perreault, JP .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (03) :533-543