Transformations of volatile methylated selenium in soil

Microbial volatilization of selenium as dimethylselenide (DMSe) and dimethyldiselenide (DMDSe) from soil is an important part of the Se cycle in nature, but little is known about the stability and transformations of these gases during residence in the soil environment before dissipation to the atmosphere. Experiments monitored by gas chromatography and atomic absorption spectroscopy were made with various clay mineral standards, charcoal, commercial humic substances and soils to determine the sorption and transformations of DMSe and DMDSe injected into the headspace or passed through soil materials. Batch experiments conducted with 2-5 g materials placed into 40 mL Teflon centrifuge tubes equipped with Mininert(R) gas sampling valves showed that DMSe was slowly sorbed by soil materials and most of the DMSe deficit in the headspace was recovered as SeO3= and Se-4(=). In contrast, DMDSe was rapidly partitioned from the gas phase and resulted in an increased recovery of less soluble elemental and selenide-Se forms. These results were confirmed during flow-through soil column studies with both little DMSe sorption and sorption of the majority of DMDSe addition. Additions of selenomethionine (SeMet) to soil to produce DMSe and DMDSe in sealed flasks resulted in an increased partitioning of Se into inorganic Se when compared with a flow-through system designed to limit the contact of Se gases with soil. These results suggest that soil Se volatilization as DMSe and DMDSe results in Se loss to the atmosphere as DMSe with concomitant soil Se immobilization due to the instability of DMDSe. (C) 1999 Elsevier Science Ltd. All rights reserved.