Studies on seed-borne mycoflora and aflatoxin B1 contaminations in food based seed samples: Molecular detection of mycotoxigenic Aspergillus flavus and their management

In the present study the mould incidence, ergosterol and aflatoxin B-1 (AFB(1)) contaminations were evaluated in different food based seed samples viz., chickpea, cowpea, green gram, groundnut, Indian bean, maize, sorghum, soya bean and sunflower collected from different agro-climatic regions of Karnataka (India). The agar plate and standard blotter methods were employed for determination of the fungal incidence, and the ergosterol and AFB(1) contents were estimated qualitatively and quantitatively by TLC and spectrophotometric methods. For detection of aflatoxigenic isolates of A. flavus, the target gene specific primer aflR-F and aflR-R were used in PCR amplification. The antifungal and antiaflatoxigenic activities of some selected edible vegetable extracts were evaluated by measuring mycelial dry weight and AFB(1) content. The results revealed that 15 diverse fungal species belonging to 11 genera were observed. Among the seed samples analyzed, the highest fungal incidence, ergosterol and AFB(1) were observed in sorghum samples followed by maize and chickpea. The PCR amplification showed positive results only for aflatoxigenic isolates of A. flavus and no amplification was observed in non-aflatoxigenic isolates and A. flavus isolate-2 produced highest AFB(1), showed 99% similarity with an authenticated aflatoxigenic A. flavus isolate emb vertical bar FN398161.1 vertical bar. The aqueous extract of Amorphophallus campanulatus showed highest antifungal and antiaflatoxigenic activities. The results confirmed that the ergosterol and AFB(1) contents were correlated with the percent mould incidences. (C) All Rights Reserved