Previsible silver staining of protein in electrophoresis gels with mass spectrometry compatibility

被引：18

作者：

Jin, Li-Tai ^{[2
]}

Li, Xiao-Kun ^{[2
]}

Cong, Wei-Tao ^{[1
]}

Hwang, Sun-Young ^{[1
]}

Choi, Jung-Kap ^{[1
]}

机构：

[1] Chonnam Natl Univ, Lab Analyt Biochem, Coll Pharm, Kwangju 500757, South Korea

[2] Wenzhou Med Coll, Sch Pharm, Wenzhou 325000, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 383卷 / 02期

关键词：

Previsible silver staining; Ethyl violet; Zincon; Proteomics; MALDI-MS;

D O I：

10.1016/j.ab.2008.04.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient silver staining method for protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels is described. The method is previsible, sensitive, and mass spectrometry (MS) compatible. Two visible counter ion dyes, ethyl violet (EV) and zincon (ZC) were used in the first staining solution with a detection limit of 2 to 8 ng/band in approximately 1 h. The dye-stained gel can be further stained by silver staining, which is based on acidic silver staining employing ZC with sodium thiosulfate as silver ion sensitizers. Especially, ZC has silver ion reducing power by cleavage of the diazo bond of the dye during silver reduction. The second silver staining can be completed in approximately 1 h with a detection limit of 0.2 ng/band. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：137 / 143

页数：7

共 34 条

[1]

Berggren K, 2000, ELECTROPHORESIS, V21, P2509, DOI 10.1002/1522-2683(20000701)21:12<2509::AID-ELPS2509>3.0.CO

[2]

2-9

[3]

Berggren KN, 2002, PROTEOMICS, V2, P486, DOI 10.1002/1615-9861(200205)2:5<486::AID-PROT486>3.0.CO

[4]

2-X

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis [J].

Candiano, G ;

Bruschi, M ;

Musante, L ;

Santucci, L ;

Ghiggeri, GM ;

Carnemolla, B ;

Orecchia, P ;

Zardi, L ;

Righetti, PG .

ELECTROPHORESIS, 2004, 25 (09) :1327-1333

[7] Proteomic capacity of recent fluorescent dyes for protein staining [J].

Chevalier, F ;

Rofidal, V ;

Vanova, P ;

Bergoin, A ;

Rossignol, M .

PHYTOCHEMISTRY, 2004, 65 (11) :1499-1506

[8] Fast visible dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels compatible with matrix assisted laser desorption/ionization-mass spectrometry [J].

Choi, JK ;

Chae, HZ ;

Hwang, SY ;

Choi, HI ;

Jin, LT ;

Yoo, GS .

ELECTROPHORESIS, 2004, 25 (7-8) :1136-1141

[9] Background-free fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes, zincon and ethyl violet [J].

Choi, JK ;

Tak, KH ;

Jin, LT ;

Hwang, SY ;

Kwon, TI ;

Yoo, GS .

ELECTROPHORESIS, 2002, 23 (24) :4053-4059

[10] SILVER STAINING OF PROTEINS IN POLYACRYLAMIDE GELS - INCREASED SENSITIVITY THROUGH A COMBINED COOMASSIE BLUE SILVER STAIN PROCEDURE [J].

DEMORENO, MR ;

SMITH, JF ;

SMITH, RV .

ANALYTICAL BIOCHEMISTRY, 1985, 151 (02) :466-470

← 1 2 3 4 →