Immunological evaluation of invasiveLactobacillus plantarumco-expressing EtMIC2 and chicken interleukin-18 againstEimeria tenella

被引:19
作者
Zhang, Zan [1 ]
Huang, Hai-Bin [1 ]
Jiang, Yan-Long [1 ]
Liu, Jing [1 ]
Gao, Xing [1 ]
Liu, Yang [1 ]
Yang, Wen-Tao [1 ]
Shi, Chun-Wei [1 ]
Wang, Dan [1 ]
Wang, Jian-Zhong [1 ]
Kang, Yuan-Huan [1 ]
Wang, Chun-Feng [1 ]
Yang, Gui-Lian [1 ]
机构
[1] Jilin Agr Univ, Minist Educ, Key Lab Anim Prod & Prod Qual Safety, Jilin Prov Engn Res Ctr Anim Probiot,Coll Anim Sc, 2888 Xincheng St, Changchun 130118, Peoples R China
基金
中国国家自然科学基金;
关键词
Coccidiosis; InvasiveLactobacillus plantarum; EtMIC2; chIL-18; DNA delivery; BINDING PROTEIN-A; DNA VACCINE; STAPHYLOCOCCUS-AUREUS; PERIPHERAL-BLOOD; CELL-SURFACE; EIMERIA; COCCIDIOSIS; INVASION; IMMUNITY; VIRUS;
D O I
10.1007/s00436-020-06745-w
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Chicken coccidiosis is a protozoan parasitic disease that leads to considerable economic losses in the poultry industry. In this study, we used invasiveLactobacillus plantarum(L.P) expressing the FnBPA protein as a novel bacterial carrier for DNA delivery into epithelial cells to develop a live oral DNA vaccine. A fusion DNA vaccine co-expressing EtMIC2 and chicken IL-18 (chIL-18) was constructed and then delivered to the host by invasiveL.P. Its efficacy againstEimeria tenellachallenge was evaluated in chickens by examining the relative weight gain rate; caecal lesion score; OPG; anti-coccidial index (ACI); levels of EtMIC2 antibody, FnBPA, IL-4, IL-18, IFN-gamma and SIgA; and proliferation ability and percentages of CD4(+)and CD8(+)splenocytes. The experimental results showed that chickens immunized with invasiveL.Pcarrying the eukaryotic expression vector pValac-EtMIC2 (pValac-EtMIC2/pSIP409-FnBPA) had markedly improved immune protection against challenge compared with that of chickens immunized with non-invasiveL.P(pValac-EtMIC2/pSIP409). However, invasiveL.Pco-expressing EtMIC2 with the chIL-18 vector exhibited the highest protection efficiency againstE. tenella. These results indicate that invasiveLactobacillus-expressing FnBPA improved humoural and cellular immunity and enhanced resistance toE. tenella. The DNA vaccine delivered by invasiveLactobacillusprovides a new concept and method for the prevention ofE. tenella.
引用
收藏
页码:2885 / 2895
页数:11
相关论文
共 35 条
  • [21] Production of Fibronectin Binding Protein A at the Surface of Lactococcus lactis Increases Plasmid Transfer In Vitro and In Vivo
    Pontes, Daniela
    Innocentin, Silvia
    del Carmen, Silvina
    Almeida, Juliana Franco
    LeBlanc, Jean-Guy
    de LeBlanc, Alejandra de Moreno
    Blugeon, Sebastien
    Cherbuy, Claire
    Lefevre, Francois
    Azevedo, Vasco
    Miyoshi, Anderson
    Langella, Philippe
    Chatel, Jean-Marc
    [J]. PLOS ONE, 2012, 7 (09):
  • [22] IMMUNITY TO COCCIDIA IN CHICKENS - ADOPTIVE TRANSFER WITH PERIPHERAL-BLOOD LYMPHOCYTES AND SPLEEN-CELLS
    ROSE, ME
    HESKETH, P
    [J]. PARASITE IMMUNOLOGY, 1982, 4 (03) : 171 - 185
  • [23] Plant expressed EtMIC2 is an effective immunogen in conferring protection against chicken coccidiosis
    Sathish, K.
    Sriraman, R.
    Subramanian, B. Mohana
    Rao, N. Hanumantha
    Balaji, K.
    Narasu, M. Lakshmi
    Srinivasan, V. A.
    [J]. VACCINE, 2011, 29 (49) : 9201 - 9208
  • [24] Chasing the golden egg: vaccination against poultry coccidiosis
    Sharman, P. A.
    Smith, N. C.
    Wallach, M. G.
    Katrib, M.
    [J]. PARASITE IMMUNOLOGY, 2010, 32 (08) : 590 - 598
  • [25] Co-expression of EtMic2 protein and chicken interleukin-18 for DNA vaccine against chicken coccidiosis
    Shi, Wenyan
    Liu, Qing
    Zhang, Jie
    Sun, Jingjing
    Jiang, Xiyue
    Geng, Jing
    Wang, Fangkun
    Xiao, Yihong
    Li, Hongmei
    Zhao, Xiaomin
    [J]. RESEARCH IN VETERINARY SCIENCE, 2014, 97 (01) : 64 - 70
  • [26] The biology of avian Eimeria with an emphasis on their control by vaccination
    Shirley, MW
    Smith, AL
    Tomley, FM
    [J]. ADVANCES IN PARASITOLOGY, VOL 60, 2005, 60 : 285 - 330
  • [27] Mechanism and consequences of invasion of endothelial cells by Staphylococcus aureus
    Sinha, B
    Herrmann, M
    [J]. THROMBOSIS AND HAEMOSTASIS, 2005, 94 (02) : 266 - 277
  • [28] The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens
    Song, Hongyan
    Qiu, Baofeng
    Yan, Ruofeng
    Xu, Lixin A.
    Song, Xiaokai
    Li, Xiangrui
    [J]. RESEARCH IN VETERINARY SCIENCE, 2013, 94 (03) : 562 - 567
  • [29] Immune protection duration and efficacy stability of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 against coccidiosis
    Song, Xiaokai
    Zhao, Xiaofang
    Xu, Lixin
    Yan, Ruofeng
    Li, Xiangrui
    [J]. RESEARCH IN VETERINARY SCIENCE, 2017, 111 : 31 - 35
  • [30] SONG XK, 2015, EXP PARASITOL, V166, P79