Differential gene expression in whitefly (Bemisia tabaci) B-biotype females and males under heat-shock condition

被引：39

作者：

Lue, Zhi-Chuang ^{[1
]}

Wan, Fang-Hao ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100081, Peoples R China

[2] Minist Agr, Ctr Management Invas Alien Species, Beijing 100081, Peoples R China

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2008年 / 3卷 / 04期

关键词：

Bemisia tabaci; Differentially expressed gene; Heat-resistance; Heat shock; Real-time PCR; Sexes; Suppression Subtractive Hybridization (SSH);

D O I：

10.1016/j.cbd.2008.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bemisia tabaci (Insecta, Hemiptera, Aleyrodidae) females are more heat resistant than males, which has important ecological significance in adaptation and expansion of B. tabaci populations. Differentially expressed genes between 25 degrees C and 44 degrees C were identified by Suppression Subtractive Hybridization (SSH) in B. tabaci sexes. 50 and 83 differentially expressed Expression Sequence Tags (EM) were obtained from female and male libraries, respectively. The ESTs have four functional categories. The frequency of heat stress-related ESTs, metabolism-related ESTs and new ESTs was higher in males than females. However, the percentage of ESTs with unclassified functions was higher in females than males. Furthermore, three differentially expressed genes were further examined by real-time PCR. The results suggested that difference of heat-resistance under heat-shock condition was associated with differentially expressed genes in B. tabaci sexes, which might enable us to better understand the mechanism behind this ecologically important trait. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：257 / 262

页数：6