Gastroprotective effects of Kangfuxin-against ethanol-induced gastric ulcer via attenuating oxidative stress and ER stress in mice

被引：40
作者：
Chen, Pianpian [1 ]
Shen, Yongmei [2 ]
Shi, Hongxue [1 ]
Ma, Xiuying [2 ]
Lin, Beibei [1 ]
Xiao, Tong [1 ]
Wu, Fenzan [3 ]
Zhu, Jingjing [1 ]
Li, Zhengmao [1 ]
Xiao, Jian [1 ]
Li, Xiaokun [1 ]
Zhang, Hongyu [1 ]
Geng, Funeng [2 ]
机构：
[1] Wenzhou Med Univ, Sch Pharmaceut Sci, Key Lab Biotechnol & Pharmaceut Engn, Wenzhou 325035, Peoples R China
[2] Sichuan Key Lab Med Amer Cockroach, Chengdu 610000, Peoples R China
[3] Wenzhou Med Univ, Cixi Peoples Hosp, Sci & Educ Div, Ningbo 315300, Zhejiang, Peoples R China
来源：
CHEMICO-BIOLOGICAL INTERACTIONS | 2016年 / 260卷
关键词：
Kangfuxin; Gastroprotection; Oxidative stress; ER stress; Apoptosis; ENDOPLASMIC-RETICULUM STRESS; SPINAL-CORD-INJURY; INDUCED APOPTOSIS; MUCOSAL INJURY; RATS; PATHWAY; PROTECTS; LESIONS; MECHANISMS; PROLIFERATION;
D O I：
10.1016/j.cbi.2016.10.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxidative stress and ER stress play a role in the pathogenesis of gastric ulcer. Kangfuxin (KFX) has been used to treat gastric ulcer in patients. However, the underlying mechanisms of KFX action remain unclear. The current study was undertaken to evaluate the gastroprotective effects of KFX and to determine its potential mechanisms. Ethanol-induced gastric ulcer mouse model was employed. Ethanol pretreated mice were treated with low (0.02 g/kg) and high (0.05 g/kg) dose of KFX for 14 days. Cimetidine (0.8 g/kg) was used as positive control. Histological evaluation of the gastric mucosa revealed that mice treated with ethanol exhibited severe gastric mucosa] damage. Ethanol treatment increased plasma and gastric MDA level, decreased plasma and gastric SOD activity, and reduced gastric HO-1 and GCL-c mRNA levels. ER stress markers (CHOP, GRP78, and caspase 12) were up-regulated upon ethanol administration. Moreover, increased cell apoptosis and pro-apoptotic protein Bax and caspase 3 were observed in ethanol treated mice, while the anti-apoptotic protein Bcl 2 was inhibited. Finally, KFX treatment reversed ethanol-induced phenotypes and ameliorated gastric ulcer. Our results demonstrated that the gastroprotective effects of KFX against ethanol-induced gastric ulcer could be attributed to its anti oxidative stress, anti-ER stress and anti-apoptotic effects. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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页码：75 / 83
页数：9
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