Sodium chloride affects propidium monoazide action to distinguish viable cells

被引：18

作者：

Barth, Valdir C., Jr. ^{[1
]}

Cattani, Fernanda ^{[1
]}

Ferreira, Carlos A. S. ^{[1
]}

de Oliveira, Silvia D. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul, Lab Imunol & Microbiol, BR-90619900 Porto Alegre, RS, Brazil

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 428卷 / 02期

关键词：

Halophiles; Archaea; PMA; Cell viability; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; QUANTITATIVE PCR; ENVIRONMENTAL-SAMPLES; STAPHYLOCOCCUS-AUREUS; ETHIDIUM MONOAZIDE; RAPID DETECTION; DNA; BACTERIA; QUANTIFICATION;

D O I：

10.1016/j.ab.2012.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Propidium monoazide (PMA) is a DNA-intercalating agent used to selectively detect DNA from viable cells by polymerase chain reaction (PCR). Here, we report that high concentrations (>5%) of sodium chloride (NaCl) prevents PMA from inhibiting DNA amplification from dead cells. Moreover, Halobacterium salinarum was unable to maintain cell integrity in solutions containing less than 15% NaCl, indicating that extreme halophilic microorganisms may not resist the concentration range in which PMA fully acts. We conclude that NaCl, but not pH, directly affects the efficiency of PMA treatment, limiting its use for cell viability assessment of halophiles and in hypersaline samples. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：108 / 110

页数：3

共 29 条

[11] Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells [J].