Studies on the activity of a bidirectional promoter of Mungbean yellow mosaic India virus by agroinfiltration

被引：17

作者：

Usharani, K. S. ^{[1
]}

Periasamy, M. ^{[1
]}

Malathi, V. G. ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Plant Pathol, Plant Virol Unit, New Delhi 110012, India

来源：

VIRUS RESEARCH | 2006年 / 119卷 / 02期

关键词：

Mungbean yellow mosaic India virus; geminivirus; Begomovirus; promoter; agroinfiltration;

D O I：

10.1016/j.virusres.2005.12.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The AV promoter expressing AV1 and AV2 genes and AC1 promoter expressing AC1 gene are present in opposite orientation in the intergenic region of Mungbean yellow mosaic India virus (MYMIV). Transient Agrobacterium-mediated delivery of putative promoter constructs into Nicotiana benthamiana and different legumes, followed by reporter gene (P-D-glucuronidase, GUS) assay, identified the promoter region of both AC1 and AV genes that is necessary for transcriptional initiation. Transcription activator protein-independent activity of AV promoter and differential regulation of AC1 promoter are unique to MYMIV. The AV promoter is a composite core promoter having both TATA box and Initiator elements (TATA(+)Inr(+)). Many transcription factor binding sites were identified in the upstream promoter sequences of both virion and complementary sense genes, which might be used in the transcription regulation studies of the host plant as well as the virus. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：154 / 162

页数：9

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