Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant

被引：3
|
作者：
Baret, P
Cesari, M
Queiroz, C
Rouch, C
Meunier, JC
Cadet, F
机构：
[1] Univ Reunion, Fac Sci & Technol, Lab Biochim & Genet Mol, F-97715 St Denis Messag 9, France
[2] Ina PG, Chaire Biochim, Chim Biol Lab, F-78850 Thiverval Grignon, France
来源：
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES | 1999年 / 322卷 / 01期
关键词：
phosphoenolpyruvate carboxylase; malate dehydrogenase; malic enzyme; Hatch and Slack cycle; multienzymatic complex; photosynthesis; C4; plants; Saccharum spp;
D O I：
10.1016/S0764-4469(99)80014-6
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have discovered a multienzymatic complex in fresh young sugarcane leaves. Th is complex is constituted of three enzymes: PEPcase, NADP-MDH and malic enzyme. After successive molecular sieving chromatography, we have obtained a highly purified sample of the complex which has a molecular weight of 711 kDa. Its functional interest has been evaluated by comparing the kinetic properties of the enzymes in their free forms to those in their complexed form. We show that the association of the three enzymes leads to important changes in their respective kinetic properties. ((C) Academie des sciences / Elsevier, Paris.).
引用
收藏
页码：29 / 34
页数：6
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