Homologous NRPS-like Gene Clusters Mediate Redundant Small-Molecule Biosynthesis in Aspergillus flavus

被引：97

作者：

Forseth, Ry R. ^{[1
,2
]}

Amaike, Saori ^{[4
]}

Schwenk, Daniel ^{[6
]}

Affeldt, Katharyn J. ^{[3
]}

Hoffmeister, Dirk ^{[6
]}

Schroeder, Frank C. ^{[1
,2
]}

Keller, Nancy P. ^{[3
,5
]}

机构：

[1] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14850 USA

[2] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14850 USA

[3] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA

[4] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA

[5] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

[6] Univ Jena, Dept Pharmaceut Biol, Hans Knoll Inst, D-07745 Jena, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 05期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

comparative metabolomics; genome mining; natural products; piperazines; secondary metabolism; IDENTIFICATION; METABOLITES; DISCOVERY; GENOMICS; ROLES; LAEA;

D O I：

10.1002/anie.201207456

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biosynthetic crosstalk: Most gene clusters in fungi are orphans with no known associated metabolites. NMR-based comparative metabolomics was used to identify the products of two highly homologous orphan clusters in Aspergillus flavus. The two clusters encode partially redundant biosynthetic pathways that produce overlapping sets of novel fungal alkaloids, feature NRPS-like genes with unusual functions, and regulate fungal development. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1590 / 1594

页数：5

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