Involvement of alpha-melanocyte stimulating hormone (α-MSH) in differential ethanol exposure and withdrawal related depression in rat:: Neuroanatomical-behavioral correlates

被引：52
作者：
Kokare, Dadasaheb M. [1 ]
Singru, Praful S. [2 ]
Dandekar, Manoj P. [1 ]
Chopde, Chandrabhan T. [1 ]
Subhedar, Nishikant K. [1 ]
机构：
[1] Rashtrasant Tukadoji Maharaj Nagpur Univ Campus, Dept Pharmaceut Sci, Nagpur 440033, Maharashtra, India
[2] Tufts Univ New England Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA 02111 USA
来源：
BRAIN RESEARCH | 2008年 / 1216卷
关键词：
alpha-melanocyte stimulating hormone; ethanol; melanocortin-4; receptor; withdrawal depression; forced swim test; immunocytochemistry;
D O I：
10.1016/j.brainres.2008.03.064
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We investigated the involvement of alpha-melanocyte stimulating hormone (alpha-MSH) following acute, chronic and withdrawal treatments of ethanol with reference to depression. The degree of depression was evaluated using Porsolt's forced swim test. While intracerebroventricular (icv) alpha-MSH (100-400 ng/rat) dose-dependently increased the immobility, opposite response was observed following administration of selective MC4 receptor antagonist HS014 (0.01-0.07 ng/rat, icv). The anti-immobility effect of acute ethanol (1-2 g/kg), injected via intra-peritoneal route (ip), was suppressed by central administration of alpha-MSH (100 ng/rat, icv), but was enhanced following pretreatment with HS014 (0.01 ng/rat, icv). Chronic ethanol resulted in increased immobility time, while further augmentation in immobility was noticed following ethanol withdrawal. However, concomitant HS014 (0.01 ng/rat, icv) treatment prevented tolerance as well as attenuated enhanced immobility in ethanol-withdrawn rats. Acute administration of HS014 (0.01-0.03 ng/rat, icv), at 24h post-withdrawal time point, also antagonized the ethanol withdrawal immobility in rats. The profile of alpha-MSH-immunoreactivity in the paraventricular (PVN), arcuate (ARC), paraventricular thalamic (PVT), dorsomedial hypothalamic-dorsal (DMNd) and -ventral (DMNv) nuclei, lateral hypothalamus (LH) and central nucleus of amygdala (CeA) was investigated with immunocytochemistry. Acute ethanol significantly reduced the alpha-MSH-immunoreactivity in the cells and fibers of ARC, and fibers in the PVN, DMNd, DMNv and CeA. While chronic ethanol treatment significantly increased the alpha-MSH-immunoreactivity as compared to the pair-fed control group, further augmentation was noticed following 24 h ethanol withdrawal. However, the alpha-MSH-immunoreactive profile in the PVT and LH did not respond. alpha-MSH in discrete areas may play a role in ethanol-induced antidepressant-like response and withdrawal-induced depression. (C) 2008 Elsevier B.V. All rights reserved.
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收藏
页码：53 / 67
页数：15
相关论文
共 79 条
[11]   MCL0042: A nonpeptidic MC4 receptor antagonist and serotonin reuptake inhibitor with anxiolytic- and antidepressant-like activity [J].
Chaki, S ;
Oshida, Y ;
Ogawa, S ;
Funakoshi, T ;
Shimazaki, T ;
Okubo, T ;
Nakazato, A ;
Okuyama, S .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 2005, 82 (04) :621-626
[12]   Involvement of the melanocortin MC4 receptor in stress-related behavior in rodents [J].
Chaki, S ;
Ogawa, S ;
Toda, Y ;
Funakoshi, T ;
Okuyama, S .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2003, 474 (01) :95-101
[13]   Anxiolytic-like and antidepressant-like activities of MCL0129 (1-[(S)-2-(4-fluorophenyl)-2-(4-isopropylpiperadin-1-yl)ethyl]-4-[4-(2-methoxynaphthalen-1-yl)butyl]piperazine), a novel and potent nonpeptide antagonist of the melanocortin-4 receptor [J].
Chaki, S ;
Hirota, S ;
Funakoshi, T ;
Suzuki, Y ;
Suetake, S ;
Okubo, T ;
Ishii, T ;
Nakazato, A ;
Okuyama, S .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2003, 304 (02) :818-826
[14]   Antidepressant-like effect of ethanol revealed in the forced swimming test in Sardinian alcohol-preferring rats [J].
Ciccocioppo, R ;
Panocka, I ;
Froldi, R ;
Colombo, G ;
Gessa, GL ;
Massi, M .
PSYCHOPHARMACOLOGY, 1999, 144 (02) :151-157
[15]   Neuropeptide Y (NPY) levels in alcoholic and food restricted male rats: implications for site selective function [J].
Clark, JT ;
Keaton, AK ;
Sahu, A ;
Kalra, SP ;
Mahajan, SC ;
Gudger, JN .
REGULATORY PEPTIDES, 1998, 75-6 :335-345
[16]   Anatomy and regulation of the central melanocortin system [J].
Cone, RD .
NATURE NEUROSCIENCE, 2005, 8 (05) :571-578
[17]   Importance of cocaine- and amphetamine-regulated transcript peptide in the central nucleus of amygdala in anxiogenic responses induced by ethanol withdrawal [J].
Dandekar, Manoj P. ;
Singru, Praful S. ;
Kokare, Dadasaheb M. ;
Lechan, Ronald M. ;
Thim, Lars ;
Clausen, Jes Thorn ;
Subhedar, Nishikant K. .
NEUROPSYCHOPHARMACOLOGY, 2008, 33 (05) :1127-1136
[18]   5-HTTLPR biases amygdala activity in response to masked facial expressions in major depression [J].
Dannlowski, Udo ;
Ohrmann, Patricia ;
Bauer, Jochen ;
Deckert, Juergen ;
Hohoff, Christa ;
Kugel, Harald ;
Arolt, Volker ;
Heindel, Walter ;
Kersting, Anette ;
Baune, Bernhard T. ;
Suslow, Thomas .
NEUROPSYCHOPHARMACOLOGY, 2008, 33 (02) :418-424
[19]   ENHANCED ACTIVITY OF THE BRAIN BETA-ENDORPHIN SYSTEM BY FREE-CHOICE ETHANOL DRINKING IN C57BL/6 BUT NOT DBA/2 MICE [J].
DEWAELE, JP ;
GIANOULAKIS, C .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1994, 258 (1-2) :119-129
[20]   The central melanocortin system and the integration of short- and long-term regulators of energy homeostasis [J].
Ellacott, KLJ ;
Cone, RD .
RECENT PROGRESS IN HORMONE RESEARCH, VOL 59, 2004, 59 :395-408
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