Cloning of an α-glucosidase gene from Thermococcus hydrothermalis by functional complementation of a Saccharomyces cerevisiae mal11n mutant strain

被引：24

作者：

Galichet, A ^{[1
]}

Belarbi, A ^{[1
]}

机构：

[1] Univ Reims, UFR Sci, Lab Microbiol Gen & Mol, F-51687 Reims 2, France

来源：

FEBS LETTERS | 1999年 / 458卷 / 02期

关键词：

alpha-glucosidase; thermophilic archaeon; mal11; mutant; Thermococcus hydrothermalis; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(99)01155-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences. (C) 1999 Federation of European Biochemical Societies.

引用

页码：188 / 192

页数：5

共 26 条

[11] Thermococcus hydrothermalis sp. nov, a new hyperthermophilic Archaeon isolated from a deep-sea hydrothermal vent [J].

Godfroy, A ;

Lesongeur, F ;

Raguenes, G ;

Querellou, J ;

Antoine, E ;

Meunier, JR ;

Guezennec, J ;

Barbier, G .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (03) :622-626

[12] A 10-MINUTE DNA PREPARATION FROM YEAST EFFICIENTLY RELEASES AUTONOMOUS PLASMIDS FOR TRANSFORMATION OF ESCHERICHIA-COLI [J].

HOFFMAN, CS ;

WINSTON, F .

GENE, 1987, 57 (2-3) :267-272

[13] A RAPID BOILING METHOD FOR THE PREPARATION OF BACTERIAL PLASMIDS [J].

HOLMES, DS ;

QUIGLEY, M .

ANALYTICAL BIOCHEMISTRY, 1981, 114 (01) :193-197

[14] Cloning, sequencing, characterization, and expression of an extracellular alpha-amylase from the hyperthermophilic archaeon Pyrococcus furiosus in Escherichia coli and Bacillus subtilis [J].

Jorgensen, S ;

Vorgias, CE ;

Antranikian, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (26) :16335-16342

[15]

LADERMAN KA, 1993, J BIOL CHEM, V268, P24402

[16] Production of thermostable amylolytic enzymes by Thermococcus hydrothermalis [J].

Legin, E ;

Copinet, A ;

Duchiron, F .

BIOTECHNOLOGY LETTERS, 1998, 20 (04) :363-367

[17]

LEGIN E, 1997, THESIS U REIMS

[18]

Linke B., 1992, DECHEMA BIOTECHNOLOG, V5, P161

[19] Properties and stabilization of an extracellular alpha-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN1: Enzyme activity at 130 degrees C [J].

Piller, K ;

Daniel, PM ;

Petach, HH .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1996, 1292 (01) :197-205

[20] Aspartate transcarbamylase from the deep-sea hyperthermophilic archaeon Pyrococcus abyssi: Genetic organization, structure, and expression in Escherichia coli [J].

Purcarea, C ;

Herve, G ;

Ladjimi, MM ;

Cunin, R .

JOURNAL OF BACTERIOLOGY, 1997, 179 (13) :4143-4157

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