Cloning of an α-glucosidase gene from Thermococcus hydrothermalis by functional complementation of a Saccharomyces cerevisiae mal11n mutant strain

被引：24

作者：

Galichet, A ^{[1
]}

Belarbi, A ^{[1
]}

机构：

[1] Univ Reims, UFR Sci, Lab Microbiol Gen & Mol, F-51687 Reims 2, France

来源：

FEBS LETTERS | 1999年 / 458卷 / 02期

关键词：

alpha-glucosidase; thermophilic archaeon; mal11; mutant; Thermococcus hydrothermalis; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(99)01155-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences. (C) 1999 Federation of European Biochemical Societies.

引用

页码：188 / 192

页数：5

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