A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis
被引:81
作者:
Amicucci, Matthew J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Amicucci, Matthew J.
[1
,2
,3
]
Nandita, Eshani
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Nandita, Eshani
[2
,3
]
Galermo, Ace G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Galermo, Ace G.
[2
,3
]
Castillo, Juan Jose
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Castillo, Juan Jose
[2
,3
]
Chen, Siyu
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Chen, Siyu
[2
,3
]
Park, Dayoung
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Park, Dayoung
[2
,3
]
Smilowitz, Jennifer T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Food Sci & Technol, Davis, CA USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Smilowitz, Jennifer T.
[3
,4
]
German, J. Bruce
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Food Sci & Technol, Davis, CA USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
German, J. Bruce
[3
,4
]
Mills, David A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Food Sci & Technol, Davis, CA USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Mills, David A.
[3
,4
]
Lebrilla, Carlito B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Lebrilla, Carlito B.
[1
,2
,3
,5
]
机构:
[1] Univ Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
[3] Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
[4] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA USA
[5] Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions. Polysaccharides cannot be simply sequenced because they are often highly branched and lack a uniform structure. Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins. While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion. We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides. The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput. The technique is first used to identify polysaccharides by oligosaccharide fingerprinting. Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies. While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by oligosaccharide fingerprinting.
引用
收藏
页数:12
相关论文
共 69 条
[1]
ALBERSHEIM PETER, 1967, CARBOHYD RES, V5, P340, DOI 10.1016/S0008-6215(00)80510-8
机构:
Univ Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Amicucci, Matthew J.
;
Galermo, Ace G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Galermo, Ace G.
;
Nandita, Eshani
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Nandita, Eshani
;
Vo, Thai-Thanh T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Vo, Thai-Thanh T.
;
Liu, Yiyun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Liu, Yiyun
;
Lee, Megan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Lee, Megan
;
Xu, Gege
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Xu, Gege
;
Lebrilla, Carlito B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
机构:
Washington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Washington Univ, Ctr Gut Microbiome & Nutr Res, St Louis, MO 63110 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Barratt, Michael J.
;
Lebrilla, Carlito
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Lebrilla, Carlito
;
Shapiro, Howard-Yana
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Coll Agr & Environm Sci, Dept Plant Sci, Davis, CA 95616 USA
World Agroforestry Ctr, Nairobi 00100, KenyaWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Shapiro, Howard-Yana
;
Gordon, Jeffrey I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Washington Univ, Ctr Gut Microbiome & Nutr Res, St Louis, MO 63110 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Bindels, Laure B.
;
Delzenne, Nathalie M.
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Delzenne, Nathalie M.
;
Cani, Patrice D.
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Cani, Patrice D.
;
Walter, Jens
论文数: 0引用数: 0
h-index: 0
机构:
Univ Alberta, Dept Agr Food & Nutrit Sci, Edmonton, AB T6G 2R3, CanadaCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
机构:
Univ Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Amicucci, Matthew J.
;
Galermo, Ace G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Galermo, Ace G.
;
Nandita, Eshani
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Nandita, Eshani
;
Vo, Thai-Thanh T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Vo, Thai-Thanh T.
;
Liu, Yiyun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Liu, Yiyun
;
Lee, Megan
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Lee, Megan
;
Xu, Gege
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
Xu, Gege
;
Lebrilla, Carlito B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, One Shields Ave, Davis, CA 95616 USA
Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA
Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USAUniv Calif Davis, Agr & Environm Chem Grad Grp, Davis, CA 95616 USA
机构:
Washington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Washington Univ, Ctr Gut Microbiome & Nutr Res, St Louis, MO 63110 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Barratt, Michael J.
;
Lebrilla, Carlito
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Chem, Davis, CA 95616 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Lebrilla, Carlito
;
Shapiro, Howard-Yana
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Coll Agr & Environm Sci, Dept Plant Sci, Davis, CA 95616 USA
World Agroforestry Ctr, Nairobi 00100, KenyaWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Shapiro, Howard-Yana
;
Gordon, Jeffrey I.
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
Washington Univ, Ctr Gut Microbiome & Nutr Res, St Louis, MO 63110 USAWashington Univ, Ctr Genome Sci & Syst Biol, St Louis, MO 63110 USA
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Bindels, Laure B.
;
Delzenne, Nathalie M.
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Delzenne, Nathalie M.
;
Cani, Patrice D.
论文数: 0引用数: 0
h-index: 0
机构:
Catholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, BelgiumCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium
Cani, Patrice D.
;
Walter, Jens
论文数: 0引用数: 0
h-index: 0
机构:
Univ Alberta, Dept Agr Food & Nutrit Sci, Edmonton, AB T6G 2R3, CanadaCatholic Univ Louvain, Metab & Nutr Res Grp, Louvain Drug Res Inst, B-1200 Brussels, Belgium