Cell-specific delivery of genes with glycosylated carriers

被引:150
作者
Hashida, M [1 ]
Nishikawa, M [1 ]
Yamashita, F [1 ]
Takakura, Y [1 ]
机构
[1] Kyoto Univ, Dept Drug Delivery Res, Grad Sch Pharmaceut Sci, Sakyo Ku, Kyoto 6068501, Japan
关键词
plasmid DNA delivery; galactosylated liposomes; galactosylated polymers; hepatocyte specific delivery; mannosylated carriers; gene therapy;
D O I
10.1016/S0169-409X(01)00209-5
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Cationic liposomes and polymers have been accepted as effective non-viral vectors for gene delivery with low immunogenicity unlike viral vectors. However, the lack of organ or cell specificity sometimes hampers their application and the development of a cell-specific targeting technology for them attracts great interest in gene therapy. In this review, the, potential of cell-specific delivery of genes with glycosylated liposomes or polymers is discussed. Galactosylated liposomes and poly(amino acids) are selectively taken up by the asialoglycoprotein receptor-positive liver parenchymal cells in vitro and in vivo after intravenous injection. DNA-galactosylated cationic liposome complexes show higher DNA uptake and gene expression in the liver parencymal cells in vitro than DNA complexes with bare cationic liposomes. In the in vitro gene transfer experiment, galactosylated liposome complexes are more efficient than DNA-galactosylated poly(amino acids) complexes but they have some difficulties in their biodistribution control. On the other hand, introduction of mannose residues to carriers resulted in specific delivery of genes to non-parenchymal liver cells. These results suggest advantages of these glycosylated carriers in cell-specific targeted delivery of genes. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:187 / 196
页数:10
相关论文
共 61 条
[1]  
Akamatsu K, 1999, J PHARMACOL EXP THER, V290, P1242
[2]   CARBOHYDRATE-SPECIFIC RECEPTORS OF THE LIVER [J].
ASHWELL, G ;
HARFORD, J .
ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :531-554
[3]   Lipoplex-mediated gene delivery to the lung occurs within 60 minutes of intravenous administration [J].
Barron, LG ;
Gagné, L ;
Szoka, FC .
HUMAN GENE THERAPY, 1999, 10 (10) :1683-1694
[4]   A NOVEL GENE DELIVERY SYSTEM USING EGF RECEPTOR-MEDIATED ENDOCYTOSIS [J].
CHEN, JB ;
GAMOU, S ;
TAKAYANAGI, A ;
SHIMIZU, N .
FEBS LETTERS, 1994, 338 (02) :167-169
[5]   Lactose-poly(ethylene glycol)-grafted poly-L-lysine as hepatoma cell-targeted gene carrier [J].
Choi, YH ;
Liu, F ;
Park, JS ;
Kim, SW .
BIOCONJUGATE CHEMISTRY, 1998, 9 (06) :708-718
[6]   Gene transfer by DNA/glycosylated polylysine complexes into human blood monocyte-derived macrophages [J].
Erbacher, P ;
Bousser, MT ;
Raimond, J ;
Monsigny, M ;
Midoux, P ;
Roche, AC .
HUMAN GENE THERAPY, 1996, 7 (06) :721-729
[7]   THE ROLE OF DIOLEOYL PHOSPHATIDYLETHANOLAMINE IN CATIONIC LIPOSOME-MEDIATED GENE-TRANSFER [J].
FARHOOD, H ;
SERBINA, N ;
HUANG, L .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1235 (02) :289-295
[8]   EFFECT OF CATIONIC CHOLESTEROL DERIVATIVES ON GENE-TRANSFER AND PROTEIN-KINASE-C ACTIVITY [J].
FARHOOD, H ;
BOTTEGA, R ;
EPAND, RM ;
HUANG, L .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1111 (02) :239-246
[9]  
FELGNER JH, 1994, J BIOL CHEM, V269, P2550
[10]   GENE-TRANSFER INTO RESPIRATORY EPITHELIAL-CELLS BY TARGETING THE POLYMERIC IMMUNOGLOBULIN RECEPTOR [J].
FERKOL, T ;
KAETZEL, CS ;
DAVIS, PB .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (05) :2394-2400