New amperometric and potentiometric immunosensors for anti-human immunoglobulin G determinations

被引：41

作者：

Campanella, L ^{[1
]}

Attioli, R ^{[1
]}

Colapicchioni, C ^{[1
]}

Tomassetti, M ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1999年 / 55卷 / 01期

关键词：

electrochemical immunosensors; anti-HIgG analysis;

D O I：

10.1016/S0925-4005(99)00035-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two new electrochemical immunosensors for the determination of antibodies such as anti-human immunoglobulin G (anti-HIgG) were fabricated and characterized. The two immunosensors investigated made use of two different types of electrochemical transducer: an amperometric electrode for H2O2 determination and a gaseous diffusion potentiometric electrode for NH3 determination. The development of these immunosensors involved the immobilization of human immunoglobulin G (HIgG) on a polymeric membrane located on the electrode head. Furthermore, two different enzymes were used as markers, thus allowing two different anti-HIgG conjugates with urease and glucose oxidase, respectively, to be obtained. Two different polymeric membranes on which the HIgG were immobilized, namely 'Pall-Biodyne' and 'Immobilon', were also employed. A method for re-utilizing the membrane of the immunosensor in which the conjugate was immobilized was also optimized. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：23 / 32

页数：10

共 16 条

[1] IMMUNOENZYMATIC ASSAY USING CHEMFET DEVICES
COLAPICCHIONI, C
BARBARO, A
PORCELLI, F
GIANNINI, I
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1991, 4 (3-4) : 245 - 250
[2] Analytical performance testing of an atrazine immunoassay system
Comber, SDW
Watts, CD
Young, B
[J]. ANALYST, 1996, 121 (10) : 1485 - 1488
[3] DEALWIS WU, 1985, ANAL CHEM, V57, P2754
[4] Engvall E, 1980, Methods Enzymol, V70, P419
[5] HOMOGENEOUS POTENTIOMETRIC ENZYME-IMMUNOASSAY FOR HUMAN IMMUNOGLOBULIN-G
FONONG, T
RECHNITZ, GA
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (13) : 2586 - 2590
[6] FOULDS NC, 1990, IMMUNOELECTRODES, P97
[7] A PIEZOELECTRIC IMMUNOBIOSENSOR FOR ATRAZINE IN DRINKING-WATER
GUILBAULT, GG
HOCK, B
SCHMID, R
[J]. BIOSENSORS & BIOELECTRONICS, 1992, 7 (06) : 411 - 419
[8] BIOANALYSIS WITH SURFACE-PLASMON RESONANCE
LOFAS, S
MALMQVIST, M
RONNBERG, I
STENBERG, E
LIEDBERG, B
LUNDSTROM, I
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1991, 5 (1-4) : 79 - 84
[9] AMPEROMETRIC SENSOR FOR PYRUVATE WITH IMMOBILIZED PYRUVATE OXIDASE
MASCINI, M
MAZZEI, F
[J]. ANALYTICA CHIMICA ACTA, 1987, 192 (01) : 9 - 16
[10] Morgan CL, 1996, CLIN CHEM, V42, P193

← 1 2 →