New amperometric and potentiometric immunosensors for anti-human immunoglobulin G determinations

被引：41

作者：

Campanella, L ^{[1
]}

Attioli, R ^{[1
]}

Colapicchioni, C ^{[1
]}

Tomassetti, M ^{[1
]}

机构：

[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1999年 / 55卷 / 01期

关键词：

electrochemical immunosensors; anti-HIgG analysis;

D O I：

10.1016/S0925-4005(99)00035-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two new electrochemical immunosensors for the determination of antibodies such as anti-human immunoglobulin G (anti-HIgG) were fabricated and characterized. The two immunosensors investigated made use of two different types of electrochemical transducer: an amperometric electrode for H2O2 determination and a gaseous diffusion potentiometric electrode for NH3 determination. The development of these immunosensors involved the immobilization of human immunoglobulin G (HIgG) on a polymeric membrane located on the electrode head. Furthermore, two different enzymes were used as markers, thus allowing two different anti-HIgG conjugates with urease and glucose oxidase, respectively, to be obtained. Two different polymeric membranes on which the HIgG were immobilized, namely 'Pall-Biodyne' and 'Immobilon', were also employed. A method for re-utilizing the membrane of the immunosensor in which the conjugate was immobilized was also optimized. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：23 / 32

页数：10

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