Antitissue transglutaminase antibodies outside celiac disease

被引:61
作者
Clemente, MG [1 ]
Musu, MP [1 ]
Frau, F [1 ]
Lucia, C [1 ]
De Virgiliis, S [1 ]
机构
[1] Univ Cagliari, Dipartimento Sci Biomed & Biotecnol, Pediat Clin 2, Ctr Reg Microcitemie, I-09121 Cagliari, Italy
关键词
autoantibody; tissue transglutaminase; celiac disease; autoimmune liver disease;
D O I
10.1097/00005176-200201000-00008
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Tissue transglutaminase enzyme-linked immunosorbent assay (tTG-ELISA) has recently been proposed as a simple and fast screening test for celiac disease (CD). The rate of false-positive and false-negative tests with tTG-ELISA, however, has not been definitively established. Therefore, the aim of our study was to investigate anti-tTG antibodies (TGA) not only in untreated patients with CD and in healthy controls, but also in a large group of patients with other autoimmune diseases. Methods: The presence of TGA was investigated in sera from I I I patients with untreated CD, 96 patients with other autoimmune conditions (28 with autoimmune liver disease, 46 with insulin-dependent diabetes mellitus, 10 with inflammatory bowel syndrome, 12 with type I polyglandular syndrome) and from 100 healthy controls using guinea pic, tTG-ELISA (gp-TG/ELISA) and highly purified recombinant human tTG-ELISA (h-TG/ELISA). Western blotting with guinea pig tTG was also performed. Results: Ninety-four patients with CD who tested positive for antiendomysial antibodies (AEA) and one who tested negative for AEA showed antibodies against the gp-TG, Among the controls, 50% of patients with autoimmune liver disease and 6.5% of patients with insulin-dependent diabetes mellitus tested positive with gp-TG/ELISA. Western blotting experiments revealed that the high rate of positive tests observed using ELISA among the control group sera is attributable to impurities in the op-TG preparation. However, h-TG/ELISA tests were positive for the sera from all patients who tested positive for AEA and from one control who tested negative for AEA, whereas h-TG/ELISA tests were negative for all CD patients who tested negative for AEA and for other controls who tested negative for AEA. Conclusions: The frequency of false-negative and false-positive tests represents the major limit to the use of gp-TG/ELISA. However, because h-TG/ELISA is both simple and fast, it could be used in large screening programs for CD.
引用
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页码:31 / 34
页数:4
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