The odorant-binding protein from Canis familiaris:: Purification, characterization and new perspectives in biohazard assessment

被引:13
作者
D'Auria, S
Staiano, M
Varriale, A
Scognamiglio, V
Rossi, M
Parracino, A
Campopiano, S
Cennamo, N
Zeni, L [1 ]
机构
[1] Univ Naples 2, Dept Informat Engn, Aversa, Italy
[2] Univ Perugia, I-06100 Perugia, Italy
[3] CNR, Inst Prot Biochem, I-80125 Naples, Italy
[4] INFM, Naples, Italy
关键词
biosensors; odorant-binding proteins; refractive index measurements; biohazard;
D O I
10.2174/092986606775974393
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this report we show the purification to homogeneity and a partial characterization of a new odorant-binding protein from Canis familiar (CfOBP) nasal mucosa. In addition, we report preliminary data on the utilization of CfOBP as a probe for the development of a refractive index-based biosensor.
引用
收藏
页码:349 / 352
页数:4
相关论文
共 23 条
  • [1] Planar antiresonant reflecting optical waveguides as integrated optical refractometer
    Bernini, R
    Campopiano, S
    de Boer, C
    Sarro, PM
    Zeni, L
    [J]. IEEE SENSORS JOURNAL, 2003, 3 (05) : 652 - 657
  • [2] Design and analysis of an integrated antiresonant reflecting optical waveguide refractive-index sensor
    Bernini, R
    Campopiano, S
    Zeni, L
    [J]. APPLIED OPTICS, 2002, 41 (01) : 70 - 73
  • [3] BERNINI R, 2003, EUR 2003 GUIM PORT
  • [4] BERNINI R, 2001, SPIE P PHOT BOST MA
  • [5] BERNINI R, 2002, IEEE SENS 2002 ORL F
  • [6] The three-dimensional structure of bovine odorant binding protein and its mechanism of odor recognition
    Blanchet, MA
    Bains, G
    Pelosi, P
    Pevsner, J
    Snyder, SH
    Monaco, HL
    Amzel, LM
    [J]. NATURE STRUCTURAL BIOLOGY, 1996, 3 (11): : 934 - 939
  • [7] CALDARARU M, 1995, P INT C SEM SIN OCT, P449
  • [8] COLE AT, 1995, ANAL BIOCHEM, P231
  • [9] A protein biosensor for lactate
    D'Auria, S
    Gryczynski, Z
    Gryczynski, I
    Rossi, M
    Lakowicz, JR
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 283 (01) : 83 - 88
  • [10] The lipocalin protein family: structural and sequence overview
    Flower, DR
    North, ACT
    Sansom, CE
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1482 (1-2): : 9 - 24