1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA

被引:12
作者
Du, Zhenming [1 ]
Liu, Yangzhong [2 ]
Zheng, Yuchuan [2 ]
McCallum, Scott [1 ]
Dansereau, John [3 ]
Derbyshire, Victoria [3 ]
Belfort, Marlene [3 ]
Belfort, Georges [1 ]
Van Roey, Patrick [3 ]
Wang, Chunyu [1 ]
机构
[1] Rensselaer Polytech Inst, Dept Biol, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
[2] Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
[3] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12208 USA
基金
美国国家卫生研究院;
关键词
Intein; Assignments; NMR; RecA; Mycobacterium tuberculosis;
D O I
10.1007/s12104-008-9098-4
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The backbone and side chain resonance assignments of an engineered intein based on Mycobacterium tuberculosis RecA have been determined based on triple-resonance experiments with the uniformly [(13)C,(15)N]-labeled protein.
引用
收藏
页码:111 / 113
页数:3
相关论文
共 9 条
[1]   Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution [J].
Derbyshire, V ;
Wood, DW ;
Wu, W ;
Dansereau, JT ;
Dalgaard, JZ ;
Belfort, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (21) :11466-11471
[2]   Minimization and stabilization of the Mycobacterium tuberculosis recA intein [J].
Hiraga, K ;
Derbyshire, V ;
Dansereau, JT ;
Van Roey, P ;
Belfort, M .
JOURNAL OF MOLECULAR BIOLOGY, 2005, 354 (04) :916-926
[3]   NMR structure of a KlbA intein precursor from Methanococcus jannaschii [J].
Johnson, Margaret A. ;
Southworth, Maurice W. ;
Herrmann, Torsten ;
Brace, Lear ;
Perler, Francine B. ;
Wuthrich, Kurt .
PROTEIN SCIENCE, 2007, 16 (07) :1316-1328
[4]  
Moure CM, 2005, NUCL ACID M, V16, P257
[5]   Protein splicing and related forms of protein autoprocessing [J].
Paulus, H .
ANNUAL REVIEW OF BIOCHEMISTRY, 2000, 69 :447-496
[6]   PROTEIN SPLICING ELEMENTS - INTEINS AND EXTEINS - A DEFINITION OF TERMS AND RECOMMENDED NOMENCLATURE [J].
PERLER, FB ;
DAVIS, EO ;
DEAN, GE ;
GIMBLE, FS ;
JACK, WE ;
NEFF, N ;
NOREN, CJ ;
THORNER, J ;
BELFORT, M .
NUCLEIC ACIDS RESEARCH, 1994, 22 (07) :1125-1127
[7]   InBase: The Intein Database [J].
Perler, FB .
NUCLEIC ACIDS RESEARCH, 2002, 30 (01) :383-384
[8]   Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction [J].
Romanelli, A ;
Shekhtman, A ;
Cowburn, D ;
Muir, TW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (17) :6397-6402
[9]   Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue [J].
Van Roey, Patrick ;
Pereira, Brian ;
Li, Zhong ;
Hiraga, Kaori ;
Belfort, Marlene ;
Derbyshire, Victoria .
JOURNAL OF MOLECULAR BIOLOGY, 2007, 367 (01) :162-173