Caspase-3 activation and increased procollagen type I in irradiated hearts

被引：9
作者：
Ferreira-Machado, Samara C. [1 ,2 ,3 ]
Salata, Camila [3 ]
Rocha, Nazareth N. [4 ]
Correa, Alexandre F. S. [5 ]
Corte-Real, Suzana [5 ]
Peregrino, Antonio A. F. [3 ]
De Campos, Vera M. A. [3 ]
Andrade, Cherley B. V. [3 ]
Bernardo-Filho, Mario [6 ]
Cabral-Neto, Januario B. [2 ]
Dealmeida, Carlos E. [3 ]
机构：
[1] Univ Fed Fluminense, Inst Biol, Dept Biol Geral, BR-24020140 Niteroi, RJ, Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Radiobiol Mol, Ilha Fundao,CCS, BR-21949900 Rio De Janeiro, RJ, Brazil
[3] Univ Estado Rio de Janeiro, Inst Biol, Dept Biofis & Biometria, LCR, BR-20550900 Rio De Janeiro, RJ, Brazil
[4] Univ Fed Fluminense, Ctr Ciencias Med, BR-24210130 Niteroi, RJ, Brazil
[5] Fiocruz MS, Inst Oswaldo Cruz, BR-21040360 Rio De Janeiro, RJ, Brazil
[6] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Lab Radiofarm Expt, BR-20551030 Rio De Janeiro, RJ, Brazil
来源：
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2013年 / 85卷 / 01期
关键词：
apoptosis; caspase-3; fibrosis; heart; ionizing radiation; TGF-beta; 1; ANGIOTENSIN-II; CARDIOMYOCYTE APOPTOSIS; ADJUVANT RADIOTHERAPY; PRESSURE-OVERLOAD; CARDIAC MYOCYTES; BREAST-CANCER; CELL-DEATH; RAT-HEART; RADIATION; MECHANISMS;
D O I：
10.1590/S0001-37652013005000009
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-beta 1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-beta 1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
引用
收藏
页码：215 / 222
页数：8
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