Cytotoxic bufadienolides from the whole plants of Helleborus foetidus

被引：13

作者：

Yokosuka, Akihito ^{[1
]}

Iguchi, Tomoki ^{[1
]}

Kawahata, Riko ^{[1
]}

Mimaki, Yoshihiro ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Dept Med Pharmacognosy, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan

来源：

PHYTOCHEMISTRY LETTERS | 2018年 / 23卷

关键词：

Bufadienolides; Bufadienolide glucosides; Helleborus foetidus; Ranunculaceae; HL-60; cells; A549; Apoptosis; SPIROSTANOL GLYCOSIDES; ORIENTALIS; RHIZOMES;

D O I：

10.1016/j.phytol.2017.10.017

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two new bufadienolides (1 and 2) and three known bufadienolide glucosides (3-5) were isolated from the whole plants of Helleborus foetidus (Ranunculaceae). The structures of 1 and 2 were determined spectroscopically. Compound 1 displayed potent cytotoxicity against HL-60 and A549 cells, with IC50 values of 0.035 and 0.029 mu M, respectively. HL-60 cells treated with 1displayed typical characteristics of apoptosis, such as nuclear chromatin condensation, DNA fragmentation, accumulation of sub-G1 cells, and marked activation of caspase-3. Moreover, the loss of mitochondrial membrane potential and release of cytochrome c into the cytosol suggested that 1 induced HL-60 cell death via the mitochondrial-dependent apoptotic pathway.

引用

页码：94 / 99

页数：6

共 13 条

[1] Mitochondrial control of apoptosis:: the role of cytochrome c [J].