Fluorometric determination of heparin based on self-quenching of fluorescein-labeled protamine

被引：41

作者：

Egawa, Yuya ^{[1
,2
]}

Hayashida, Ryosuke ^{[1
]}

Seki, Toshinobu ^{[2
]}

Anzai, Jun-ichi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Josai Univ, Fac Pharmaceut Sci, Sakado, Saitama 3500295, Japan

来源：

TALANTA | 2008年 / 76卷 / 04期

关键词：

fluorescein; fluorescence; heparin; protamine; self-quenching;

D O I：

10.1016/j.talanta.2008.04.019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new signaling technique for a fluorometric heparin assay has been developed using the self-quenching of fluoresceinisothiocyanate-labeled protamine (F-protamine). The binding between F-protamine and heparin led to a significant fluorescence quenching due to enhancing the proximity of the F-protamine molecules. The fluorescence of F-protamine (5.9 mu g/mL) decreased to 13% in the presence of 2.0 mu g/mL heparin. An advantage of this self-quenching system is that the detection can be carried out without using other indicators. With the present system, we could monitor the therapeutic level of heparin in bovine plasma, and the response was quick. These properties of the self-quenching system are suitable for practical use. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：736 / 741

页数：6

共 24 条

[1] Probing BSA binding to citrate-coated gold nanoparticles and surfaces [J].