Prevalence of a Novel Division-Level Bacterial Lineage in Lake Dhanmondi, Dhaka, Bangladesh, as Revealed by Deep Sequencing of 16S rRNA Gene Amplicons

被引：4

作者：

Azmuda, Nafisa ^{[1
,2
,3
]}

Rahman, Mohammed Ziaur ^{[3
]}

Madsen, Marit Steine ^{[1
,2
]}

Khan, Sirajul Islam ^{[3
]}

Birkeland, Nils-Kare ^{[1
,2
]}

机构：

[1] Univ Bergen, Dept Biol, N-5020 Bergen, Norway

[2] Univ Bergen, Ctr Geobiol, N-5020 Bergen, Norway

[3] Univ Dhaka, Dept Microbiol, Dhaka 1000, Bangladesh

来源：

CURRENT MICROBIOLOGY | 2012年 / 65卷 / 04期

关键词：

Freshwater bacterial division; Lake Dhanmondi; LD11; SHIGELLA-FLEXNERI; DIVERSITY;

D O I：

10.1007/s00284-012-0165-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A culture-independent study of the bacterial diversity in Lake Dhanmondi, located in the central region of Dhaka city, Bangladesh, was carried out using deep sequence analysis of 16S rRNA gene PCR amplicons. The results revealed the presence of a group of bacteria, termed LD11, phylogenetically unrelated to any previously cultivated bacteria at the phylum level. LD11 sequences comprised about 1.7 % of the total sequence reads after quality assessment. LD11 appears to constitute a novel division with a deep evolutionary lineage apparently branching between the Chloroflexi and Thermi-Deinococci phyla. Sequence similarity with molecular data from freshwater environments indicates that LD11 represents a widespread and novel clade of freshwater bacteria for which no cultivated representatives are yet available.

引用

页码：356 / 360

页数：5

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