The Effect of ADAM8 on the Proliferation and Apoptosis of Hepatocytes and Hepatoma Carcinoma Cells

被引：3

作者：

Li, San-Qiang ^{[1
]}

Hu, Zhi-Hong ^{[1
]}

Zhu, Sha ^{[2
]}

Wang, Dong-Mei ^{[1
]}

Han, Hong-Mei ^{[1
]}

Lu, Hua-Jie ^{[1
]}

机构：

[1] Henan Univ Sci & Technol, Coll Med, Mol Med Key Lab Liver Injury & Repair, Luoyang 471003, Peoples R China

[2] Zhengzhou Univ, Coll Basic Med Sci, Dept Microbiol Immunol, Zhengzhou 45001, Peoples R China

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2015年 / 29卷 / 09期

基金：

中国国家自然科学基金;

关键词：

ADAM8; Proliferation; Apoptosis; METALLOPROTEASE-DISINTEGRIN ADAM8; OSTEOCLAST-STIMULATING FACTOR; HEPATOCELLULAR-CARCINOMA; NUCLEAR ANTIGEN; EXPRESSION; PCNA; TISSUE;

D O I：

10.1002/jbt.21737

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was undertaken to evaluate the effect of ADAM8 on the proliferation and apoptosis of hepatocytes and hepatoma carcinoma cells during hepatocellular carcinoma (HCC) progression. The expression of ADAM8 was significantly increased with good correlation of PCNA expression increasing and cells apoptosis decreasing during the progression of HCC in the liver of mice. Proliferation experiment in vitro showed that recombinant ADAM8 could induce the expression of PCNA in L02 cells, but not in HepG2 cells. Apoptosis experiment in vitro showed that recombinant ADAM8 did not induce or inhibit the expression of apoptosis-related factors Bcl2, Bax, and Caspase3 in L02 cells, but significantly induced the expression of Bcl2, inhibited the expression of Bax and Caspase3 in HepG2 cells. In conclusion, our study suggested that ADAM8 could promote the proliferation of normal hepatocytes and render hepatoma carcinoma cells more resistant to apoptosis to play important roles during the progression of HCC. ADAM8; Proliferation; Apoptosis

引用

页码：440 / 448

页数：9

共 23 条

[1] Use of proliferating cell nuclear antigen as a marker of liver regeneration after partial hepatectomy in rats
Assy, N
Gong, Y
Zhang, M
Pettigrew, NM
Pashniak, D
Minuk, GY
[J]. JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 1998, 131 (03): : 251 - 256
[2] ADAM8: A novel osteoclast stimulating factor
Choi, SJ
Han, JH
Roodman, GD
[J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2001, 16 (05) : 814 - 822
[3] Dhanasekaran R, 2012, HEPATIC MED-EVID RES, V4, P19, DOI [10.1586/egh.09.35, 10.2147/HMER.S16316]
[4] Microarray analysis of peroxisome proliferator-activated receptor γ-induced changes in gene expression in macrophages
Hodgkinson, CP
Ye, S
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 308 (03) : 505 - 510
[5] Hoshi T, 1998, ANTICANCER RES, V18, P4347
[6] Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice
Kelly, K
Hutchinson, G
Nebenius-Oosthuizen, D
Smith, AJH
Bartsch, JW
Horiuchi, K
Rittger, A
Manova, K
Docherty, AJP
Blobel, CP
[J]. DEVELOPMENTAL DYNAMICS, 2005, 232 (01) : 221 - 231
[7] PCNA: Structure, functions and interactions
Kelman, Z
[J]. ONCOGENE, 1997, 14 (06) : 629 - 640
[8] ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies
Koller, Garrit
Schlomann, Uwe
Golfi, Panagiota
Ferdous, Taheera
Naus, Silvia
Bartsch, Joerg W.
[J]. CURRENT PHARMACEUTICAL DESIGN, 2009, 15 (20) : 2272 - 2281
[9] Hepatocellular carcinoma: prevention, detection and treatment ... in the real world
Koorey, D.
[J]. INTERNAL MEDICINE JOURNAL, 2007, 37 (08) : 513 - 515
[10] Lau WY, 2008, HEPATOB PANCREAT DIS, V7, P237

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