De Novo Design of Ln(III) Coiled Coils for Imaging Applications

被引：54

作者：

Berwick, Matthew R. ^{[1
]}

Lewis, David J. ^{[1
]}

Jones, Andrew W. ^{[2
]}

Parslow, Rosemary A. ^{[2
]}

Dafforn, Timothy R. ^{[2
]}

Cooper, Helen J. ^{[2
]}

Wilkie, John ^{[1
]}

Pikramenou, Zoe ^{[1
]}

Britton, Melanie M. ^{[1
]}

Peacock, Anna F. A. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Edgbaston B15 2TT, W Midlands, England

[2] Univ Birmingham, Sch Biosci, Edgbaston B15 2TT, W Midlands, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

MRI CONTRAST AGENTS; PROTEIN DESIGN; BINDING; COORDINATION; PEPTIDES; METALLOPROTEIN; FLUORESCENCE;

D O I：

10.1021/ja408741h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new peptide sequence (MB1) has been designed which, in the presence of a trivalent lanthanide ion, has been programmed to self-assemble to form a three stranded metallo-coiled coil, Ln(III)(MB1)(3). The binding site has been incorporated into the hydrophobic core using natural amino acids, restricting water access to the lanthanide. The resulting terbium coiled coil displays luminescent properties consistent with a lack of first coordination sphere water molecules. Despite this the gadolinium coiled coil, the first to be reported, displays promising magnetic resonance contrast capabilities.

引用

页码：1166 / 1169

页数：4

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