Uptake of bacteriophage lambda DNA in Escherichia coli by electroporation: An alternative to in vitro packaging

被引：0
|
作者：
Gaynor, JJ
Fox, MG
机构：
来源：
BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 06期
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D O I：
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中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage lambda DNA, we obtained efficiencies of 1.1 X 10(6) (pfu/mu g bacteriophage lambda DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage lambda DNA for the production of cDNA and genomic libraries.
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页码：463 / 467
页数：5
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